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TitleBiochemical and Structural Insights into an Fe(II)/ alpha-Ketoglutarate/O2-Dependent Dioxygenase, Kdo 3-Hydroxylase (KdoO).
Journal, issue, pagesJ. Mol. Biol., Vol. 430, Page 4036-4048, Year 2018
Publish dateOct 12, 2017 (structure data deposition date)
AuthorsJoo, S.H. / Pemble, C.W. / Yang, E.G. / Raetz, C.R.H. / Chung, H.S.
External linksJ. Mol. Biol. / PubMed:30092253
MethodsX-ray diffraction
Resolution1.448 - 1.939 Å
Structure data

PDB-5yka:
Crystal structure of the Kdo hydroxylase KdoO, a non-heme Fe(II) alphaketoglutarate dependent dioxygenase in complex with cobalt(II)
Method: X-RAY DIFFRACTION / Resolution: 1.448 Å

PDB-5yvz:
Crystal structure of the Kdo hydroxylase KdoO, a non-heme Fe(II) alphaketoglutarate dependent dioxygenase in complex with alphaketoglutarate and Fe(III)
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-5yw0:
Crystal structure of the Kdo hydroxylase KdoO, a non-heme Fe(II) alphaketoglutarate dependent dioxygenase in complex with succinate and Fe(III)
Method: X-RAY DIFFRACTION / Resolution: 1.49 Å

PDB-6a2e:
Apo structure of the Kdo hydroxylase KdoO, a non-heme Fe(II) alphaketoglutarate dependent dioxygenase
Method: X-RAY DIFFRACTION / Resolution: 1.939 Å

Chemicals

ChemComp-CO:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-ACT:
ACETATE ION

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-FE:
Unknown entry

ChemComp-AKG:
2-OXOGLUTARIC ACID

ChemComp-SIN:
SUCCINIC ACID

Source
  • methylacidiphilum infernorum (isolate v4) (bacteria)
  • methylacidiphilum infernorum (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / Fe(II)/O2/alphaketoglutarate dependent dioxygenase / KDO2-lipid A dioxygenase / deoxysugar dioxygenase / LPS biosynthesis

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