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TitleExperimental phase determination with selenomethionine or mercury-derivatization in serial femtosecond crystallography
Journal, issue, pagesIUCrJ, Vol. 4, Page 639-647, Year 2017
Publish dateApr 10, 2017 (structure data deposition date)
AuthorsYamashita, K. / Kuwabara, N. / Nakane, T. / Murai, T. / Mizohata, E. / Sugahara, M. / Pan, D. / Masuda, T. / Suzuki, M. / Sato, T. ...Yamashita, K. / Kuwabara, N. / Nakane, T. / Murai, T. / Mizohata, E. / Sugahara, M. / Pan, D. / Masuda, T. / Suzuki, M. / Sato, T. / Kodan, A. / Yamaguchi, T. / Nango, E. / Tanaka, T. / Tono, K. / Joti, Y. / Kameshima, T. / Hatsui, T. / Yabashi, M. / Manya, H. / Endo, T. / Kato, R. / Senda, T. / Kato, H. / Iwata, S. / Ago, H. / Yamamoto, M. / Yumoto, F. / Nakatsu, T.
External linksIUCrJ / PubMed:28989719
MethodsX-ray diffraction
Resolution1.4 - 1.5 Å
Structure data

PDB-5xfc:
Serial femtosecond X-ray structure of a stem domain of human O-mannose beta-1,2-N-acetylglucosaminyltransferase solved by Se-SAD using XFEL (refined against 13,000 patterns)
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-5xfd:
Serial femtosecond X-ray structure of Agrocybe cylindracea galectin with lactose solved by Se-SAD using XFEL (refined against 60,000 patterns)
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5xfe:
Luciferin-regenerating enzyme solved by SAD using XFEL (refined against 11,000 patterns)
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-MBE:
4-nitrophenyl beta-D-mannopyranoside

ChemComp-HOH:
WATER

ChemComp-HG:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

Source
  • homo sapiens (human)
  • agrocybe cylindracea (poplar fieldcap)
  • photinus pyralis (common eastern firefly)
KeywordsSUGAR BINDING PROTEIN / glycosyltransferase / carbohydrate-binding domain / HYDROLASE / BETA-PROOELLER

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