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TitleHuman TRPML1 channel structures in open and closed conformations.
Journal, issue, pagesNature, Vol. 550, Issue 7676, Page 366-370, Year 2017
Publish dateOct 19, 2017
AuthorsPhilip Schmiege / Michael Fine / Günter Blobel / Xiaochun Li /
PubMed AbstractTransient receptor potential mucolipin 1 (TRPML1) is a Ca-releasing cation channel that mediates the calcium signalling and homeostasis of lysosomes. Mutations in TRPML1 lead to mucolipidosis type ...Transient receptor potential mucolipin 1 (TRPML1) is a Ca-releasing cation channel that mediates the calcium signalling and homeostasis of lysosomes. Mutations in TRPML1 lead to mucolipidosis type IV, a severe lysosomal storage disorder. Here we report two electron cryo-microscopy structures of full-length human TRPML1: a 3.72-Å apo structure at pH 7.0 in the closed state, and a 3.49-Å agonist-bound structure at pH 6.0 in an open state. Several aromatic and hydrophobic residues in pore helix 1, helices S5 and S6, and helix S6 of a neighbouring subunit, form a hydrophobic cavity to house the agonist, suggesting a distinct agonist-binding site from that found in TRPV1, a TRP channel from a different subfamily. The opening of TRPML1 is associated with distinct dilations of its lower gate together with a slight structural movement of pore helix 1. Our work reveals the regulatory mechanism of TRPML channels, facilitates better understanding of TRP channel activation, and provides insights into the molecular basis of mucolipidosis type IV pathogenesis.
External linksNature / PubMed:29019983 / PubMed Central
MethodsEM (single particle)
Resolution3.49 - 3.72 Å
Structure data

8840

5wj5

EMDB-8840, PDB-5wj5:
Human TRPML1 channel structure in closed conformation
Method: EM (single particle) / Resolution: 3.72 Å

8841

5wj9

EMDB-8841: cryo-EM of a lysosomal protein
PDB-5wj9: Human TRPML1 channel structure in agonist-bound open conformation
Method: EM (single particle) / Resolution: 3.49 Å

Chemicals

ChemComp-AQV:
2-{2-oxo-2-[(4S)-2,2,4-trimethyl-3,4-dihydroquinolin-1(2H)-yl]ethyl}-1H-isoindole-1,3(2H)-dione

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / human TRPML1

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