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Structure paper

TitleMolecular basis for the substrate specificity of quorum signal synthases.
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 114, Page 9092-9097, Year 2017
Publish dateJun 21, 2017 (structure data deposition date)
AuthorsDong, S.H. / Frane, N.D. / Christensen, Q.H. / Greenberg, E.P. / Nagarajan, R. / Nair, S.K.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:28784791
MethodsX-ray diffraction
Resolution1.8 - 2 Å
Structure data

PDB-5w8a:
The structure of a COA-dependent acyl-homoserine lactone synthase, BjaI, with SAM and isopentyl-CoA
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-5w8c:
The structure of a COA-dependent acyl-homoserine lactone synthase, BjaI, with MTA and isovaleryl-CoA
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-5w8d:
The structure of a COA-dependent acyl-homoserine lactone synthase, BjaI, with MTA
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-5w8e:
The structure of a CoA-dependent acyl-homoserine lactone synthase, BjaI, with the adduct of SAH and IV-CoA
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5w8g:
The structure of a COA-dependent acyl-homoserine lactone synthase, BjaI, with SAH
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-SAM:
S-ADENOSYLMETHIONINE

ChemComp-A1S:
isopentyl-Coenzyme A

ChemComp-HOH:
WATER

ChemComp-IVC:
Isovaleryl-coenzyme A

ChemComp-MTA:
5'-DEOXY-5'-METHYLTHIOADENOSINE

ChemComp-GOL:
GLYCEROL

ChemComp-CL:
Unknown entry

ChemComp-SXZ:
(2S)-4-({[(2S,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl}sulfanyl)-2-[(3-methylbutanoyl)amino]butanoic acid

ChemComp-ADE:
ADENINE

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-SO4:
SULFATE ION

Source
  • bradyrhizobium japonicum (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / acyl-homoserine lactone / coenzyme A / BjaI

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