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TitleAntibody-induced uncoating of human rhinovirus B14.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 114, Issue 30, Page 8017-8022, Year 2017
Publish dateJul 25, 2017
AuthorsYangchao Dong / Yue Liu / Wen Jiang / Thomas J Smith / Zhikai Xu / Michael G Rossmann /
PubMed AbstractRhinoviruses (RVs) are the major causes of common colds in humans. They have a nonenveloped, icosahedral capsid surrounding a positive-strand RNA genome. Here we report that the antigen-binding (Fab) ...Rhinoviruses (RVs) are the major causes of common colds in humans. They have a nonenveloped, icosahedral capsid surrounding a positive-strand RNA genome. Here we report that the antigen-binding (Fab) fragment of a neutralizing antibody (C5) can trigger genome release from RV-B14 to form emptied particles and neutralize virus infection. Using cryo-electron microscopy, structures of the C5 Fab in complex with the full and emptied particles have been determined at 2.3 Å and 3.0 Å resolution, respectively. Each of the 60 Fab molecules binds primarily to a region on viral protein 3 (VP3). Binding of the C5 Fabs to RV-B14 results in significant conformational changes around holes in the capsid through which the viral RNA might exit. These results are so far the highest resolution view of an antibody-virus complex and elucidate a mechanism whereby antibodies neutralize RVs and related viruses by inducing virus uncoating.
External linksProc Natl Acad Sci U S A / PubMed:28696310 / PubMed Central
MethodsEM (single particle)
Resolution2.26 - 3.01 Å
Structure data

8754

5w3e

EMDB-8754, PDB-5w3e:
CryoEM structure of rhinovirus B14 in complex with C5 Fab (33 degrees Celsius, molar ratio 1:3, full particle)
Method: EM (single particle) / Resolution: 2.53 Å

8761

5w3l

EMDB-8761, PDB-5w3l:
CryoEM structure of rhinovirus B14 in complex with C5 Fab (4 degrees Celsius, molar ratio 1:3, full particle)
Method: EM (single particle) / Resolution: 2.71 Å

8762

5w3m

EMDB-8762, PDB-5w3m:
CryoEM structure of rhinovirus B14 in complex with C5 Fab (33 degrees Celsius, molar ratio 1:1, full particle)
Method: EM (single particle) / Resolution: 2.26 Å

8763

5w3o

EMDB-8763, PDB-5w3o:
CryoEM structure of rhinovirus B14 in complex with C5 Fab (33 degrees Celsius, molar ratio 1:3, empty particle)
Method: EM (single particle) / Resolution: 3.01 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • Mouse (mice)
  • HRV-14, Human rhinovirus B14
  • mus musculus (house mouse)
  • human rhinovirus 14
KeywordsVIRUS/IMMUNE SYSTEM / virus / antibody / VIRUS-IMMUNE SYSTEM complex / VIRUS LIKE PARTICLE/IMMUNE SYSTEM / VIRUS LIKE PARTICLE-IMMUNE SYSTEM complex

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