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Structure paper

TitleCharacterization of Two Late-Stage Enzymes Involved in Fosfomycin Biosynthesis in Pseudomonads.
Journal, issue, pagesACS Chem. Biol., Vol. 12, Page 456-463, Year 2017
Publish dateDec 6, 2016 (structure data deposition date)
AuthorsOlivares, P. / Ulrich, E.C. / Chekan, J.R. / van der Donk, W.A. / Nair, S.K.
External linksACS Chem. Biol. / PubMed:27977135
MethodsX-ray diffraction
Resolution2.048 - 2.73 Å
Structure data

PDB-5u55:
Psf4 in complex with Mn2+ and (S)-2-HPP
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-5u57:
Psf4 in complex with Fe2+ and (S)-2-HPP
Method: X-RAY DIFFRACTION / Resolution: 2.73 Å

PDB-5u58:
Psf4 in complex with Fe2+ and (R)-2-HPP
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-5u5d:
Psf4 in complex with Mn2+ and (R)-2-HPP
Method: X-RAY DIFFRACTION / Resolution: 2.49 Å

PDB-5u5g:
Psf3 in complex with NADP+ and 2-OPP
Method: X-RAY DIFFRACTION / Resolution: 2.048 Å

Chemicals

ChemComp-MN:
Unknown entry

ChemComp-S0H:
(S)-2-HYDROXYPROPYLPHOSPHONIC ACID

ChemComp-HOH:
WATER

ChemComp-FE:
Unknown entry

ChemComp-TB6:
[(2R)-2-hydroxypropyl]phosphonic acid

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-7VD:
(2-oxopropyl)phosphonic acid

ChemComp-PGE:
TRIETHYLENE GLYCOL

Source
  • pseudomonas syringae (bacteria)
KeywordsOXIDOREDUCTASE / Fosfomycin / Epoxidase / Oxidase / Reductase

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