+検索条件
-Structure paper
タイトル | Cryo-EM structures of the autoinhibited ATP synthase in three rotational states. |
---|---|
ジャーナル・号・ページ | Elife, Vol. 5, Year 2016 |
掲載日 | 2016年12月21日 |
著者 | Meghna Sobti / Callum Smits / Andrew Sw Wong / Robert Ishmukhametov / Daniela Stock / Sara Sandin / Alastair G Stewart / |
PubMed 要旨 | A molecular model that provides a framework for interpreting the wealth of functional information obtained on the F-ATP synthase has been generated using cryo-electron microscopy. Three different ...A molecular model that provides a framework for interpreting the wealth of functional information obtained on the F-ATP synthase has been generated using cryo-electron microscopy. Three different states that relate to rotation of the enzyme were observed, with the central stalk's ε subunit in an extended autoinhibitory conformation in all three states. The F motor comprises of seven transmembrane helices and a decameric c-ring and invaginations on either side of the membrane indicate the entry and exit channels for protons. The proton translocating subunit contains near parallel helices inclined by ~30° to the membrane, a feature now synonymous with rotary ATPases. For the first time in this rotary ATPase subtype, the peripheral stalk is resolved over its entire length of the complex, revealing the F attachment points and a coiled-coil that bifurcates toward the membrane with its helices separating to embrace subunit from two sides. |
リンク | Elife / PubMed:28001127 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 6.9 - 8.53 Å |
構造データ | |
化合物 | ChemComp-ATP: ChemComp-ADP: |
由来 |
|
キーワード | HYDROLASE (加水分解酵素) / ATP synthase (ATP合成酵素) / ATPase (ATPアーゼ) / rotary motor (電動機) / membrane protein (膜タンパク質) |