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Structure paper

TitlepH driven fibrillar aggregation of the super-sweet protein Y65R-MNEI: A step-by-step structural analysis.
Journal, issue, pagesBiochim. Biophys. Acta, Vol. 1862, Page 808-815, Year 2017
Publish dateJun 9, 2017 (structure data deposition date)
AuthorsPica, A. / Leone, S. / Di Girolamo, R. / Donnarumma, F. / Emendato, A. / Rega, M.F. / Merlino, A. / Picone, D.
External linksBiochim. Biophys. Acta / PubMed:29288772
MethodsX-ray diffraction
Resolution1.72 - 2.6 Å
Structure data

PDB-5o7k:
Crystal structure of a single chain monellin mutant (Y65R) pH 2.0
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-5o7l:
Crystal structure of a single chain monellin mutant (Y65R) pH 4.6
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-5o7q:
Crystal structure of a single chain monellin mutant (Y65R) pH 5.5
Method: X-RAY DIFFRACTION / Resolution: 1.72 Å

PDB-5o7r:
Crystal structure of a single chain monellin mutant (Y65R) pH 6.5
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

PDB-5o7s:
Crystal structure of a single chain monellin mutant (Y65R) pH 8.3
Method: X-RAY DIFFRACTION / Resolution: 2.02 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • dioscoreophyllum cumminsii (serendipity berry)
KeywordsPLANT PROTEIN / SWEET PROTEIN / SWEETENER

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