Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	The Complete Structure of the Mycobacterium smegmatis 70S Ribosome.
Journal, issue, pages	Cell Rep, Vol. 20, Issue 1, Page 149-160, Year 2017
Publish date	Jul 5, 2017
Authors	Jendrik Hentschel / Chloe Burnside / Ingrid Mignot / Marc Leibundgut / Daniel Boehringer / Nenad Ban /
PubMed Abstract	The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death ...The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3.3-Å cryo-EM structure of the 70S ribosome of Mycobacterium smegmatis, a close relative to the human pathogen Mycobacterium tuberculosis. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization. Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics.
External links	Cell Rep / PubMed:28683309
Methods	EM (single particle)
Resolution	3.18 - 3.45 Å
Structure data	3748 5o5j EMDB-3748: Cryo-EM reconstruction of the small subunit of the Mycobacterium smegmatis ribosome PDB-5o5j: Structure of the 30S small ribosomal subunit from Mycobacterium smegmatis Method: EM (single particle) / Resolution: 3.45 Å 3750 5o60 EMDB-3750: Cryo-EM reconstruction of the large subunit of the Mycobacterium smegmatis ribosome PDB-5o60: Structure of the 50S large ribosomal subunit from Mycobacterium smegmatis Method: EM (single particle) / Resolution: 3.18 Å 3751 5o61 EMDB-3751: Cryo-EM reconstruction of the Mycobacterium smegmatis 70S ribosome PDB-5o61: The complete structure of the Mycobacterium smegmatis 70S ribosome Method: EM (single particle) / Resolution: 3.31 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-PHE: PHENYLALANINE
Source	mycobacterium smegmatis str. mc2 155 (bacteria)
Keywords	RIBOSOME / translation