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Structure paper

TitleZampanolide Binding to Tubulin Indicates Cross-Talk of Taxane Site with Colchicine and Nucleotide Sites.
Journal, issue, pagesJ. Nat. Prod., Vol. 81, Page 494-505, Year 2018
Publish dateMar 16, 2017 (structure data deposition date)
AuthorsField, J.J. / Pera, B. / Gallego, J.E. / Calvo, E. / Rodriguez-Salarichs, J. / Saez-Calvo, G. / Zuwerra, D. / Jordi, M. / Andreu, J.M. / Prota, A.E. ...Field, J.J. / Pera, B. / Gallego, J.E. / Calvo, E. / Rodriguez-Salarichs, J. / Saez-Calvo, G. / Zuwerra, D. / Jordi, M. / Andreu, J.M. / Prota, A.E. / Menchon, G. / Miller, J.H. / Altmann, K.H. / Diaz, J.F.
External linksJ. Nat. Prod. / PubMed:29023132
MethodsX-ray diffraction
Resolution2.1 - 2.2 Å
Structure data

PDB-5nfz:
TUBULIN-MTC complex
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-5ng1:
TUBULIN-MTC-zampanolide complex
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-8WB:
2-methoxy-5-(2,3,4-trimethoxyphenyl)cyclohepta-2,4,6-trien-1-one

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-ACP:
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogue*YM

ChemComp-HOH:
WATER

ChemComp-8WE:
(2~{Z},4~{E})-~{N}-[(~{S})-oxidanyl-[(1~{S},2~{E},5~{S},11~{R},17~{S},19~{R})-3,11,19-trimethyl-7,13-bis(oxidanylidene)-6,21-dioxabicyclo[15.3.1]henicos-2-en-5-yl]methyl]hexa-2,4-dienamide

ChemComp-ZPN:
(2Z,4E)-N-[(S)-[(1S,2E,5S,8E,10Z,17S)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide

Source
  • rattus norvegicus (Norway rat)
  • gallus gallus (chicken)
  • bos taurus (domestic cattle)
KeywordsCELL CYCLE / TUBULIN FOLD / CYTOSKELETON / MICROTUBULE

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