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Structure paper

TitleBiosynthesis of methyl-proline containing griselimycins, natural products with anti-tuberculosis activity.
Journal, issue, pagesChem Sci, Vol. 8, Page 7521-7527, Year 2017
Publish dateMar 5, 2017 (structure data deposition date)
AuthorsLukat, P. / Katsuyama, Y. / Wenzel, S. / Binz, T. / Konig, C. / Blankenfeldt, W. / Bronstrup, M. / Muller, R.
External linksChem Sci / PubMed:29163906
MethodsX-ray diffraction
Resolution1.529 - 1.819 Å
Structure data

PDB-5nch:
GriE apo form
Method: X-RAY DIFFRACTION / Resolution: 1.819 Å

PDB-5nci:
GriE in complex with cobalt, alpha-ketoglutarate and l-leucine
Method: X-RAY DIFFRACTION / Resolution: 1.755 Å

PDB-5ncj:
GriE in complex with manganese, succinate and (2S,4R)-5-hydroxyleucine
Method: X-RAY DIFFRACTION / Resolution: 1.529 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-CO:
Unknown entry

ChemComp-AKG:
2-OXOGLUTARIC ACID

ChemComp-LEU:
LEUCINE

ChemComp-NA:
Unknown entry

ChemComp-MN:
Unknown entry

ChemComp-SIN:
SUCCINIC ACID

ChemComp-HL5:
(2S,4R)-5-hydroxyleucine

Source
  • streptomyces sp. dsm 40835 (bacteria)
KeywordsOXIDOREDUCTASE / Fe(II)/alpha-ketoglutarate dependent dioxygenase / hydroxylase / non-heme iron protein / leucine hydroxylase / 5-hydroxyleucine / (2S4R)-5-hydroxyleucine / 4-methyl-proline / griselimycin / methyl-griselimycin

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