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Structure paper

TitleaPKC Inhibition by Par3 CR3 Flanking Regions Controls Substrate Access and Underpins Apical-Junctional Polarization.
Journal, issue, pagesDev. Cell, Vol. 38, Page 384-398, Year 2016
Publish dateJul 13, 2016 (structure data deposition date)
AuthorsSoriano, E.V. / Ivanova, M.E. / Fletcher, G. / Riou, P. / Knowles, P.P. / Barnouin, K. / Purkiss, A. / Kostelecky, B. / Saiu, P. / Linch, M. ...Soriano, E.V. / Ivanova, M.E. / Fletcher, G. / Riou, P. / Knowles, P.P. / Barnouin, K. / Purkiss, A. / Kostelecky, B. / Saiu, P. / Linch, M. / Elbediwy, A. / Kjr, S. / O'Reilly, N. / Snijders, A.P. / Parker, P.J. / Thompson, B.J. / McDonald, N.Q.
External linksDev. Cell / PubMed:27554858
MethodsX-ray diffraction
Resolution1.79 - 3.25 Å
Structure data

PDB-5li1:
Structure of a Par3-inhibitory peptide bound to PKCiota core kinase domain
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-5li9:
Structure of a nucleotide-bound form of PKCiota core kinase domain
Method: X-RAY DIFFRACTION / Resolution: 1.79 Å

PDB-5lih:
Structure of a peptide-substrate bound to PKCiota core kinase domain
Method: X-RAY DIFFRACTION / Resolution: 3.25 Å

Chemicals

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-GOL:
GLYCEROL

ChemComp-K:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-ACP:
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogue*YM

ChemComp-FMT:
FORMIC ACID

ChemComp-IMD:
IMIDAZOLE

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-ACT:
ACETATE ION

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-AF3:
ALUMINUM FLUORIDE

ChemComp-MN:
Unknown entry

ChemComp-SCN:
THIOCYANATE ION

Source
  • homo sapiens (human)
  • xenopus tropicalis (tropical clawed frog)
KeywordsTRANSFERASE / aPKC / Polarity / Complex

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