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TitleTransition state mimics are valuable mechanistic probes for structural studies with the arginine methyltransferase CARM1.
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 114, Page 3625-3630, Year 2017
Publish dateJul 8, 2016 (structure data deposition date)
Authorsvan Haren, M.J. / Marechal, N. / Troffer-Charlier, N. / Cianciulli, A. / Sbardella, G. / Cavarelli, J. / Martin, N.I.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:28330993
MethodsX-ray diffraction
Resolution2 - 2.11 Å
Structure data

PDB-5lgp:
Crystal structure of mouse CARM1 in complex with ligand P1C3s
Method: X-RAY DIFFRACTION / Resolution: 2.04 Å

PDB-5lgq:
Crystal structure of mouse CARM1 in complex with ligand P2C3s
Method: X-RAY DIFFRACTION / Resolution: 2.11 Å

PDB-5lgr:
Crystal structure of mouse CARM1 in complex with ligand P1C3u
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-5lgs:
Crystal structure of mouse CARM1 in complex with ligand P2C3u
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-8ZB:
(2~{R},3~{R},4~{S},5~{R})-2-(6-aminopurin-9-yl)-5-propyl-oxolane-3,4-diol

ChemComp-LPD:
L-PROLINAMIDE

ChemComp-HOH:
WATER

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-DXE:
1,2-DIMETHOXYETHANE

ChemComp-PG6:
1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE

ChemComp-SO4:
SULFATE ION

ChemComp-QVR:
(2~{R},3~{R},4~{S},5~{R})-2-(6-aminopurin-9-yl)-5-[(~{E})-prop-1-enyl]oxolane-3,4-diol

ChemComp-ACE:
ACETYL GROUP

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

Source
  • mus musculus (house mouse)
  • homo sapiens (human)
KeywordsTRANSFERASE / PROTEIN ARGININE METHYLTRANSFERASE / CATALYTIC DOMAIN / CHROMATIN REGULATOR / MRNA PROCESSING / MRNA SPLICING / NUCLEUS / S-ADENOSYL-L-METHIONINE / TRANSCRIPTION / TRANSCRIPTION REGULATION

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