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Structure paper

TitleMechanism of extracellular ion exchange and binding-site occlusion in a sodium/calcium exchanger
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 23, Page 590-599, Year 2016
Publish dateJan 29, 2016 (structure data deposition date)
AuthorsLiao, J. / Marinelli, F. / Lee, C. / Huang, Y. / Faraldo-Gomez, J.D. / Jiang, Y.
External linksNat. Struct. Mol. Biol. / PubMed:27183196
MethodsX-ray diffraction
Resolution1.897 - 2.904 Å
Structure data

PDB-5hwx:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 2.5 mM Na+ and zero Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5hwy:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 10 mM Na+ and zero Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.098 Å

PDB-5hxc:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 20 mM Na+ and zero Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.101 Å

PDB-5hxe:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 100 mM Na+ and zero Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.288 Å

PDB-5hxh:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with zero Na+ and Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.804 Å

PDB-5hxr:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 2.5 mM Na+ and 10mM Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.463 Å

PDB-5hxs:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 2.5 mM Na+ and 10mM Sr2+
Method: X-RAY DIFFRACTION / Resolution: 2.789 Å

PDB-5hya:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchangerNCX_Mj soaked with 150 mM Na+ and nominal Ca2+
Method: X-RAY DIFFRACTION / Resolution: 1.897 Å

PDB-5jdf:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 2.5 mM Na+ and 1mM Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-5jdg:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 2.5 mM Na+ and 0.1mM Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.407 Å

PDB-5jdh:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 10 mM Na+ and 10mM Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.203 Å

PDB-5jdl:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 2.5 mM Na+ and 1mM Sr2+
Method: X-RAY DIFFRACTION / Resolution: 2.904 Å

PDB-5jdm:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 2.5 mM Na+ and 0.1mM Sr2+
Method: X-RAY DIFFRACTION / Resolution: 2.558 Å

PDB-5jdn:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 10 mM Na+ and 10mM Sr2+
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-5jdq:
Structural mechanisms of extracellular ion exchange and induced binding-site occlusion in the sodium-calcium exchanger NCX_Mj soaked with 100 mM Na+ and 10mM Sr2+
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-MYS:
PENTADECANE

ChemComp-HOH:
WATER

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-ACT:
ACETATE ION

ChemComp-CA:
Unknown entry

ChemComp-MYC:
3,5,7-TRIHYDROXY-2-(3,4,5-TRIHYDROXYPHENYL)-4H-CHROMEN-4-ONE

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-SR:
STRONTIUM ION

ChemComp-TOE:
2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL

ChemComp-PE8:
3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL

ChemComp-P3G:
3,6,9,12,15-PENTAOXAHEPTADECANE

Source
  • methanocaldococcus jannaschii (strain atcc 43067 / dsm 2661 / jal-1 / jcm 10045 / nbrc 100440) (archaea)
  • methanocaldococcus jannaschii (archaea)
KeywordsMEMBRANE PROTEIN / Na+/Ca2+ exchange / Calcium signalling / membrane transporter / induced conformational change

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