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Structure paper

TitleStructural Basis of Backwards Motion in Kinesin-1-Kinesin-14 Chimera: Implication for Kinesin-14 Motility.
Journal, issue, pagesStructure, Vol. 24, Issue 8, Page 1322-1334, Year 2016
Publish dateAug 2, 2016
AuthorsMasahiko Yamagishi / Hideki Shigematsu / Takeshi Yokoyama / Masahide Kikkawa / Mitsuhiro Sugawa / Mari Aoki / Mikako Shirouzu / Junichiro Yajima / Ryo Nitta /
PubMed AbstractKinesin-14 is a unique minus-end-directed microtubule-based motor. A swinging motion of a class-specific N-terminal neck helix has been proposed to produce minus-end directionality. However, it is ...Kinesin-14 is a unique minus-end-directed microtubule-based motor. A swinging motion of a class-specific N-terminal neck helix has been proposed to produce minus-end directionality. However, it is unclear how swinging of the neck helix is driven by ATP hydrolysis utilizing the highly conserved catalytic core among all kinesins. Here, using a motility assay, we show that in addition to the neck helix, the conserved five residues at the C-terminal region in kinesin-14, namely the neck mimic, are necessary to give kinesin-1 an ability to reverse its directionality toward the minus end of microtubules. Our structural analyses further demonstrate that the C-terminal neck mimic, in cooperation with conformational changes in the catalytic core during ATP binding, forms a kinesin-14 bundle with the N-terminal neck helix to swing toward the minus end of microtubules. Thus, the neck mimic plays a crucial role in coupling the chemical ATPase reaction with the mechanical cycle to produce the minus-end-directed motility of kinesin-14.
External linksStructure / PubMed:27452403
MethodsEM (helical sym.)
Resolution5.8 - 6.6 Å
Structure data

8058

5hnw

EMDB-8058, PDB-5hnw:
Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the AMPPNP state
Method: EM (helical sym.) / Resolution: 6.6 Å

8059

5hnx

EMDB-8059, PDB-5hnx:
Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the nucleotide-free state
Method: EM (helical sym.) / Resolution: 6.6 Å

8060

5hny

EMDB-8060, PDB-5hny:
Structural basis of backwards motion in kinesin-14: plus-end directed nKn669 in the AMPPNP state
Method: EM (helical sym.) / Resolution: 6.3 Å

8061

5hnz

EMDB-8061, PDB-5hnz:
Structural basis of backwards motion in kinesin-14: plus-end directed nKn669 in the nucleotide-free state
Method: EM (helical sym.) / Resolution: 5.8 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM / Paclitaxel

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

Source
  • rattus norvegicus (Norway rat)
  • bos taurus (cattle)
  • drosophila melanogaster (fruit fly)
  • Bovine (cattle)
KeywordsTRANSPORT PROTEIN / kinesin / kinesin-14 / microtubule / ATPase / STRUCTURAL PROTEIN/MOTOR PROTEIN / STRUCTURAL PROTEIN-MOTOR PROTEIN complex

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