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Structure paper

TitleCrystal Structures of the Bspif1 Helicase Reveal that a Major Movement of the 2B SH3 Domain is Required for DNA Unwinding
Journal, issue, pagesNucleic Acids Res., Vol. 44, Page 2949-, Year 2016
Publish dateJan 12, 2016 (structure data deposition date)
AuthorsChen, W.-F. / Dai, Y.-X. / Duan, X.-L. / Liu, N.-N. / Shi, W. / Li, N. / Li, M. / Dou, S.-X. / Dong, Y.-H. / Rety, S. / Xi, X.-G.
External linksNucleic Acids Res. / PubMed:26809678
MethodsX-ray diffraction
Resolution1.38 - 3.19 Å
Structure data

PDB-5ftb:
Crystal structure of Pif1 helicase from Bacteroides in complex with AMPPNP
Method: X-RAY DIFFRACTION / Resolution: 1.38 Å

PDB-5ftc:
Crystal structure of Pif1 helicase from Bacteroides in complex with ADP
Method: X-RAY DIFFRACTION / Resolution: 2.269 Å

PDB-5ftd:
Crystal structure of Pif1 helicase from Bacteroides apo form
Method: X-RAY DIFFRACTION / Resolution: 1.695 Å

PDB-5fte:
Crystal structure of Pif1 helicase from Bacteroides in complex with ADP-AlF3 and ssDNA
Method: X-RAY DIFFRACTION / Resolution: 3.19 Å

PDB-5ftf:
Crystal structure of Pif1 helicase from Bacteroides double mutant L95C-I339C
Method: X-RAY DIFFRACTION / Resolution: 2.412 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-CA:
Unknown entry

ChemComp-PO4:
PHOSPHATE ION

ChemComp-AF3:
ALUMINUM FLUORIDE

Source
  • bacteroides (bacteria)
  • synthetic construct (others)
KeywordsHYDROLASE / SF1B / G QUADRUPLEX / SH3 DOMAIN / CONFORMATIONAL CHANGE / DISULPHIDE BRIDGE

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