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Structure paper

TitleThe Structure of the Transcriptional Repressor KstR in Complex with CoA Thioester Cholesterol Metabolites Sheds Light on the Regulation of Cholesterol Catabolism in Mycobacterium tuberculosis.
Journal, issue, pagesJ. Biol. Chem., Vol. 291, Page 7256-7266, Year 2016
Publish dateApr 21, 2010 (structure data deposition date)
AuthorsHo, N.A. / Dawes, S.S. / Crowe, A.M. / Casabon, I. / Gao, C. / Kendall, S.L. / Baker, E.N. / Eltis, L.D. / Lott, J.S.
External linksJ. Biol. Chem. / PubMed:26858250
MethodsX-ray diffraction
Resolution1.8 - 2.6 Å
Structure data

PDB-3mnl:
The crystal structure of KstR (Rv3574) from Mycobacterium tuberculosis H37Rv
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5cw8:
Crystal structure of Mycobacterium tuberculosis KstR in complex with 3-oxo-4-cholesten-26-oyl-CoA
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-5cxg:
Crystal structure of Mycobacterium tuberculosis KstR in complex with PEG
Method: X-RAY DIFFRACTION / Resolution: 2.1001 Å

PDB-5cxi:
Crystal structure of Mycobacterium tuberculosis KstR in complex with 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA (4-BNC-CoA)
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-HOH:
WATER

ChemComp-55X:
S-{1-[5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,7-dihydroxy-6,6-dimethyl-3-oxido-8,12

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-5TW:
3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA

Source
  • mycobacterium tuberculosis (bacteria)
  • mycobacterium tuberculosis (strain atcc 25618 / h37rv) (bacteria)
KeywordsTRANSCRIPTION REGULATOR / tetR family of transcriptional regulator / all-helical / DNA-binding / hydrophobic ligand-binding / Structural Genomics / TB Structural Genomics Consortium / TBSGC / Transcriptional repressor / TetR family Transcriptional repressor / cholesterol catabolism / CoA thioester ligand / TRANSCRIPTION / TetR family Tcholesterol catabolism

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