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-Structure paper
Title | Ribosome. The complete structure of the 55S mammalian mitochondrial ribosome. |
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Journal, issue, pages | Science, Vol. 348, Issue 6232, Page 303-308, Year 2015 |
Publish date | Apr 17, 2015 |
![]() | Basil J Greber / Philipp Bieri / Marc Leibundgut / Alexander Leitner / Ruedi Aebersold / Daniel Boehringer / Nenad Ban / ![]() |
PubMed Abstract | Mammalian mitochondrial ribosomes (mitoribosomes) synthesize mitochondrially encoded membrane proteins that are critical for mitochondrial function. Here we present the complete atomic structure of ...Mammalian mitochondrial ribosomes (mitoribosomes) synthesize mitochondrially encoded membrane proteins that are critical for mitochondrial function. Here we present the complete atomic structure of the porcine 55S mitoribosome at 3.8 angstrom resolution by cryo-electron microscopy and chemical cross-linking/mass spectrometry. The structure of the 28S subunit in the complex was resolved at 3.6 angstrom resolution by focused alignment, which allowed building of a detailed atomic structure including all of its 15 mitoribosomal-specific proteins. The structure reveals the intersubunit contacts in the 55S mitoribosome, the molecular architecture of the mitoribosomal messenger RNA (mRNA) binding channel and its interaction with transfer RNAs, and provides insight into the highly specialized mechanism of mRNA recruitment to the 28S subunit. Furthermore, the structure contributes to a mechanistic understanding of aminoglycoside ototoxicity. |
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Methods | EM (single particle) |
Resolution | 3.6 - 3.8 Å |
Structure data | |
Chemicals | ![]() ChemComp-MG: ![]() ChemComp-ZN: ![]() ChemComp-GDP: ![]() ChemComp-HOH: |
Source |
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![]() | RIBOSOME / TRANSLATION / MITOCHONDRIA / MAMMALIAN 55S MITORIBOSOME / MAMMALIAN 55S MITOCHONDRIAL RIBOSOME / 28S SMALL SUBUNIT / MRNA / TRNA / DECODING CENTER / CRYO-EM / SINGLE PARTICLE ANALYSIS / 39S LARGE SUBUNIT / PTC / CRYO- EM |