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TitleGeneration of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools.
Journal, issue, pagesJ. Mol. Biol., Vol. 428, Page 1272-1289, Year 2016
Publish dateJul 27, 2015 (structure data deposition date)
AuthorsSchutz, M. / Batyuk, A. / Klenk, C. / Kummer, L. / de Picciotto, S. / Gulbakan, B. / Wu, Y. / Newby, G.A. / Zosel, F. / Schoppe, J. ...Schutz, M. / Batyuk, A. / Klenk, C. / Kummer, L. / de Picciotto, S. / Gulbakan, B. / Wu, Y. / Newby, G.A. / Zosel, F. / Schoppe, J. / Sedlak, E. / Mittl, P.R. / Zenobi, R. / Wittrup, K.D. / Pluckthun, A.
External linksJ. Mol. Biol. / PubMed:26812208
MethodsX-ray diffraction
Resolution2.6 Å
Structure data

PDB-5aao:
Crystal structure of fluorogen-activating designed ankyrin repeat protein (DARPin) dimer in complex with malachite green
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-6FE:
4-[[4-(dimethylamino)cyclohexa-2,5-dien-1-ylidene]-(4-methoxyphenyl)methyl]-N,N-dimethyl-aniline

ChemComp-HOH:
WATER

Source
  • synthetic construct (others)
KeywordsFLUORESCENT PROTEIN / HOMODIMER / DESIGNED ANKYRIN REPEAT PROTEIN (DARPIN) / FLUOROGEN-ACTIVATION / MALACHITE GREEN

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