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Structure paper

TitleStructure of the Ergothioneine-Biosynthesis Amidohydrolase EgtC.
Journal, issue, pagesChembiochem, Vol. 16, Page 1490-1496, Year 2015
Publish dateApr 21, 2015 (structure data deposition date)
AuthorsVit, A. / Mashabela, G.T. / Blankenfeldt, W. / Seebeck, F.P.
External linksChembiochem / PubMed:26079795
MethodsX-ray diffraction
Resolution1.7 - 1.82 Å
Structure data

PDB-4zfj:
Ergothioneine-biosynthetic Ntn hydrolase EgtC, apo form
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-4zfk:
Ergothioneine-biosynthetic Ntn hydrolase EgtC with glutamine
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

PDB-4zfl:
Ergothioneine-biosynthetic Ntn hydrolase variant EgtC_C2A with natural substrate
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-XPE:
3,6,9,12,15,18,21,24,27-NONAOXANONACOSANE-1,29-DIOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-GLN:
GLUTAMINE

ChemComp-4NK:
(1S)-1-carboxy-4-({(1R)-1-carboxy-2-[(S)-{4-[(2S)-2-carboxy-2-(trimethylammonio)ethyl]-1H-imidazol-2-yl}sulfinyl]ethyl}amino)-4-oxobutan-1-aminium

ChemComp-GOL:
GLYCEROL

Source
  • mycobacterium smegmatis (strain atcc 700084 / mc(2)155) (bacteria)
  • mycobacterium smegmatis (bacteria)
KeywordsHYDROLASE / Ntn hydrolase / ergothioneine biosynthesis / sulfur chemistry / Mycobacteria

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