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Title | Study of the structural dynamics of the E coli 70S ribosome using real-space refinement. |
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Journal, issue, pages | Cell, Vol. 113, Issue 6, Page 789-801, Year 2003 |
Publish date | Jun 13, 2003 |
Authors | Haixiao Gao / Jayati Sengupta / Mikel Valle / Andrei Korostelev / Narayanan Eswar / Scott M Stagg / Patrick Van Roey / Rajendra K Agrawal / Stephen C Harvey / Andrej Sali / Michael S Chapman / Joachim Frank / |
PubMed Abstract | Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting ...Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation. |
External links | Cell / PubMed:12809609 |
Methods | EM (single particle) |
Resolution | 11.5 - 12.3 Å |
Structure data | PDB-4v47: PDB-4v48: |
Source |
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Keywords | RIBOSOME / EF-G.GTP-bound state / ratchet-like movement / real-space refinement |