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-Structure paper
| タイトル | Structural virology. Near-atomic cryo-EM structure of the helical measles virus nucleocapsid. |
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| ジャーナル・号・ページ | Science, Vol. 348, Issue 6235, Page 704-707, Year 2015 |
| 掲載日 | 2015年5月8日 |
著者 | Irina Gutsche / Ambroise Desfosses / Grégory Effantin / Wai Li Ling / Melina Haupt / Rob W H Ruigrok / Carsten Sachse / Guy Schoehn / ![]() |
| PubMed 要旨 | Measles is a highly contagious human disease. We used cryo-electron microscopy and single particle-based helical image analysis to determine the structure of the helical nucleocapsid formed by the ...Measles is a highly contagious human disease. We used cryo-electron microscopy and single particle-based helical image analysis to determine the structure of the helical nucleocapsid formed by the folded domain of the measles virus nucleoprotein encapsidating an RNA at a resolution of 4.3 angstroms. The resulting pseudoatomic model of the measles virus nucleocapsid offers important insights into the mechanism of the helical polymerization of nucleocapsids of negative-strand RNA viruses, in particular via the exchange subdomains of the nucleoprotein. The structure reveals the mode of the nucleoprotein-RNA interaction and explains why each nucleoprotein of measles virus binds six nucleotides, whereas the respiratory syncytial virus nucleoprotein binds seven. It provides a rational basis for further analysis of measles virus replication and transcription, and reveals potential targets for drug design. |
リンク | Science / PubMed:25883315 |
| 手法 | EM (単粒子) |
| 解像度 | 4.3 Å |
| 構造データ | |
| 由来 |
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キーワード | RNA BINDING PROTEIN / MEASLES VIRUS NUCLEOCAPSID / TRANSCRIPTION AND REPLICATION TEMPLATE |
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