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TitleGleaning Unexpected Fruits from Hard-Won Synthetases: Probing Principles of Permissivity in Non-canonical Amino Acid-tRNA Synthetases.
Journal, issue, pagesChembiochem, Vol. 15, Page 1810-1819, Year 2014
Publish dateApr 13, 2014 (structure data deposition date)
AuthorsCooley, R.B. / Karplus, P.A. / Mehl, R.A.
External linksChembiochem / PubMed:25044993
MethodsX-ray diffraction
Resolution1.9 - 2.011 Å
Structure data

PDB-4pbr:
Crystal structure of the M. jannaschii G2 tRNA synthetase variant bound to 4-(2-bromoisobutyramido)-phenylalanine (BibaF)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4pbs:
Crystal structure of the M. jannaschii F9 tRNA synthetase variant bound to 4-(2-bromoisobutyramido)-phenylalanine (BibaF)
Method: X-RAY DIFFRACTION / Resolution: 2.011 Å

PDB-4pbt:
Crystal structure of the M. jannaschii G2 tRNA synthetase variant bound to 4-trans-cyclooctene-amidopheylalanine (Tco-amF)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-2L7:
4-[(2-bromo-2-methylpropanoyl)amino]-L-phenylalanine

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-P4G:
1-ETHOXY-2-(2-ETHOXYETHOXY)ETHANE

ChemComp-2LQ:
4-{[(1R,4E)-cyclooct-4-en-1-ylcarbonyl]amino}-L-phenylalanine

Source
  • methanocaldococcus jannaschii (archaea)
KeywordsLIGASE / tRNA synthetase / non-natural amino acid

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