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Structure paper

TitleCrystallographic and spectroscopic snapshots reveal a dehydrogenase in action.
Journal, issue, pagesNat Commun, Vol. 6, Page 5935-5935, Year 2015
Publish dateNov 21, 2012 (structure data deposition date)
AuthorsHuo, L. / Davis, I. / Liu, F. / Andi, B. / Esaki, S. / Iwaki, H. / Hasegawa, Y. / Orville, A.M. / Liu, A.
External linksNat Commun / PubMed:25565451
MethodsX-ray diffraction
Resolution1.94 - 2.204 Å
Structure data

PDB-4i1w:
2.00 Angstroms X-ray crystal structure of NAD- bound 2-aminomuconate 6-semialdehyde dehydrogenase from Pseudomonas fluorescens
Method: X-RAY DIFFRACTION / Resolution: 1.992 Å

PDB-4i25:
2.00 Angstroms X-ray crystal structure of NAD- and substrate-bound 2-aminomuconate 6-semialdehyde dehydrogenase from Pseudomonas fluorescens
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4i26:
2.20 Angstroms X-ray crystal structure of 2-aminomuconate 6-semialdehyde dehydrogenase from Pseudomonas fluorescens
Method: X-RAY DIFFRACTION / Resolution: 2.204 Å

PDB-4i2r:
2.15 Angstroms X-ray crystal structure of NAD- and alternative substrate-bound 2-aminomuconate 6-semialdehyde dehydrogenase from Pseudomonas fluorescens
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-4npi:
1.94 Angstroms X-ray crystal structure of NAD- and intermediate- bound alpha-aminomuconate-epsilon-semialdehyde dehydrogenase from Pseudomonas fluorescens
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

PDB-4oe2:
2.00 Angstroms X-ray crystal structure of E268A 2-aminomuconate 6-semialdehyde dehydrogenase from Pseudomonas fluorescens
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4ou2:
A 2.15 Angstroms X-ray crystal structure of E268A 2-aminomuconate 6-semialdehyde dehydrogenase catalytic intermediate from Pseudomonas fluorescens
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-4oub:
A 2.20 angstroms X-ray crystal structure of E268A 2-aminomucaonate 6-semialdehyde dehydrogenase catalytic intermediate from Pseudomonas fluorescens
Method: X-RAY DIFFRACTION / Resolution: 2.19 Å

Chemicals

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

ChemComp-6OD:
(2E,4E)-2-amino-6-oxohexa-2,4-dienoic acid

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-6OH:
(2E,4E)-2-hydroxy-6-oxohexa-2,4-dienoic acid

ChemComp-2VS:
(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoic acid

ChemComp-6OA:
(2Z,4E)-2,6-dihydroxyhexa-2,4-dienoic acid

Source
  • pseudomonas fluorescens (bacteria)
KeywordsOXIDOREDUCTASE / Aldehyde dehydrogenase / Dehydrogenase / NAD / ALDH / NAD+

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