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Structure paper

TitleA Phosphate-Binding Pocket within the Platform-PAZ-Connector Helix Cassette of Human Dicer.
Journal, issue, pagesMol. Cell, Vol. 53, Page 606-616, Year 2014
Publish dateNov 1, 2013 (structure data deposition date)
AuthorsTian, Y. / Simanshu, D.K. / Ma, J.B. / Park, J.E. / Heo, I. / Kim, V.N. / Patel, D.J.
External linksMol. Cell / PubMed:24486018
MethodsX-ray diffraction
Resolution1.958 - 3.401 Å
Structure data

PDB-4ngb:
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 12-mer siRNA having UU-3' ends (2.25 Angstrom resolution)
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-4ngc:
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 12-mer siRNA having UA-3' ends (2.1 Angstrom resolution)
Method: X-RAY DIFFRACTION / Resolution: 2.104 Å

PDB-4ngd:
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 12-mer siRNA having 5'-p and UU-3' ends (1.95 Angstrom resolution)
Method: X-RAY DIFFRACTION / Resolution: 1.958 Å

PDB-4ngf:
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 17-mer siRNA having 5'-p and UU-3' ends (3.1 Angstrom resolution)
Method: X-RAY DIFFRACTION / Resolution: 3.101 Å

PDB-4ngg:
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-4nh3:
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-pU and UU-3' ends (2.6 Angstrom resolution)
Method: X-RAY DIFFRACTION / Resolution: 2.616 Å

PDB-4nh5:
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 14-mer siRNA having 5'-pUU and UU-3' ends (2.55 Angstrom resolution)
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-4nh6:
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 15-mer siRNA having 5'-pUUU and UU-3' ends (2.55 Angstrom resolution)
Method: X-RAY DIFFRACTION / Resolution: 2.551 Å

PDB-4nha:
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 16-mer siRNA having 5'-p and UU-3' ends (3.4 Angstrom resolution)
Method: X-RAY DIFFRACTION / Resolution: 3.401 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PO4:
PHOSPHATE ION

Source
  • homo sapiens (human)
KeywordsHYDROLASE/RNA / PAZ domain / platform domain / connector helix / siRNA / RNase III domain / endoribonuclease / pre-miRNA / HYDROLASE-RNA complex

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