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Structure paper

TitlePrediction and biochemical demonstration of a catabolic pathway for the osmoprotectant proline betaine.
Journal, issue, pagesMBio, Vol. 5, Page e00933-e00913, Year 2014
Publish dateMar 20, 2012 (structure data deposition date)
AuthorsKumar, R. / Zhao, S. / Vetting, M.W. / Wood, B.M. / Sakai, A. / Cho, K. / Solbiati, J. / Almo, S.C. / Sweedler, J.V. / Jacobson, M.P. ...Kumar, R. / Zhao, S. / Vetting, M.W. / Wood, B.M. / Sakai, A. / Cho, K. / Solbiati, J. / Almo, S.C. / Sweedler, J.V. / Jacobson, M.P. / Gerlt, J.A. / Cronan, J.E.
External linksMBio / PubMed:24520058
MethodsX-ray diffraction
Resolution1.6 - 2 Å
Structure data

PDB-4e8g:
Crystal structure of an enolase (mandelate racemase subgroup) from paracococus denitrificans pd1222 (target nysgrc-012907) with bound mg
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4izg:
Crystal structure of an enolase (mandelate racemase subgroup) from paracococus denitrificans pd1222 (target nysgrc-012907) with bound cis-4oh-d-proline betaine (product)
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-4j1o:
Crystal structure of an enolase (mandelate racemase subgroup) from paracococus denitrificans pd1222 (target nysgrc-012907) with bound l-proline betaine (substrate)
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

Chemicals

ChemComp-MG:
Unknown entry


ChemComp, No image

ChemComp-UNL:
Unknown ligand

ChemComp-HOH:
WATER

ChemComp-IOD:
IODIDE ION

ChemComp-4OP:
(2S,4S)-2-carboxy-4-hydroxy-1,1-dimethylpyrrolidinium

ChemComp-GOL:
GLYCEROL

ChemComp-PBE:
1,1-DIMETHYL-PROLINIUM

Source
  • paracoccus denitrificans (bacteria)
KeywordsISOMERASE / Putative Racemase / NYSGRC / Structural Genomics / PSI-Biology / New York Structural Genomics Research Consortium / Enolase / Betaine Racemase / Proline Betaine Racemease

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