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Structure paper

TitleThe catalytic cycle for ribonucleotide incorporation by human DNA Pol lambda
Journal, issue, pagesNucleic Acids Res., Vol. 40, Page 7518-7527, Year 2012
Publish dateNov 18, 2011 (structure data deposition date)
AuthorsGosavi, R.A. / Moon, A.F. / Kunkel, T.A. / Pedersen, L.C. / Bebenek, K.
External linksNucleic Acids Res. / PubMed:22584622
MethodsX-ray diffraction
Resolution1.95 - 2.25 Å
Structure data

PDB-3upq:
Crystal structure of the pre-catalytic ternary complex of polymerase lambda with an rATP analog opposite a templating T.
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-3uq0:
Crystal structure of the post-catalytic product complex of polymerase lambda with an rAMP at the primer terminus.
Method: X-RAY DIFFRACTION / Resolution: 2.14 Å

PDB-3uq2:
Crystal structure of the post-catalytic product complex of polymerase lambda with an rCMP inserted opposite a templating G and dAMP inserted opposite a templating T at the primer terminus.
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-4fo6:
Crystal structure of the pre-catalytic ternary complex of polymerase lambda with a dATP analog opposite a templating T and an rCMP at the primer terminus.
Method: X-RAY DIFFRACTION / Resolution: 2.007 Å

Chemicals

ChemComp-ZAN:
5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]adenosine

ChemComp-MN:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PPV:
PYROPHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-F2A:
2'-deoxy-5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]adenosine

Source
  • homo sapiens (human)
KeywordsTRANSFERASE / LYASE/DNA / DNA / Polymerase / DNA polymerase lambda / ribonucleotide incorporation / LYASE-DNA complex / Protein Conformation / DNA polymerase

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