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Structure paper

TitleNucleotides Adjacent to the Ligand-Binding Pocket are Linked to Activity Tuning in the Purine Riboswitch.
Journal, issue, pagesJ. Mol. Biol., Vol. 425, Page 1596-1611, Year 2013
Publish dateMay 30, 2012 (structure data deposition date)
AuthorsStoddard, C.D. / Widmann, J. / Trausch, J.J. / Marcano-Velazquez, J.G. / Knight, R. / Batey, R.T.
External linksJ. Mol. Biol. / PubMed:23485418
MethodsX-ray diffraction
Resolution1.35 - 1.65 Å
Structure data

PDB-4fej:
Crystal structure of the A24U mutant xpt-pbuX guanine riboswitch aptamer domain in complex with hypoxanthine
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-4fel:
Crystal structure of the U25A/A46G mutant of the xpt-pbuX guanine riboswitch aptamer domain in complex with hypoxanthine
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-4fen:
Crystal structure of the A24U/U25A/A46G mutant xpt-pbuX guanine riboswitch aptamer domain in complex with hypoxanthine
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-4feo:
Crystal structure of the AU25A/A46G/C74U mutant xpt-pbuX guanine riboswitch aptamer domain in complex with 2,6-diaminopurine
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-4fep:
Crystal structure of the A24U/U25A/A46G/C74U mutant xpt-pbuX guanine riboswitch aptamer domain in complex with 2,6-diaminopurine
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

Chemicals

ChemComp-HPA:
HYPOXANTHINE

ChemComp-ACT:
ACETATE ION

ChemComp-NCO:
COBALT HEXAMMINE(III)

ChemComp-HOH:
WATER

ChemComp-6AP:
9H-PURINE-2,6-DIAMINE

KeywordsRNA / three-way junction with distal tertiary interaction / genetic regulatory element / hypoxanthine / 2 / 6-diaminopurine

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