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Structure paper

TitleOn the catalytic mechanism of dimeric dUTPases.
Journal, issue, pagesBiochem. J., Vol. 456, Page 81-88, Year 2013
Publish dateFeb 3, 2012 (structure data deposition date)
AuthorsHemsworth, G.R. / Gonzalez-Pacanowska, D. / Wilson, K.S.
External linksBiochem. J. / PubMed:24001052
MethodsX-ray diffraction
Resolution1.47 - 1.9 Å
Structure data

PDB-4dk2:
Crystal Structure of Open Trypanosoma brucei dUTPase
Method: X-RAY DIFFRACTION / Resolution: 1.47 Å

PDB-4dk4:
Crystal Structure of Trypanosoma brucei dUTPase with dUpNp, Ca2+ and Na+
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4dkb:
Crystal Structure of Trypanosoma brucei dUTPase with dUpNp and Ca2+
Method: X-RAY DIFFRACTION / Resolution: 1.831 Å

PDB-4dl8:
Crystal structure of Trypanosoma brucei dUTPase with dUMP, planar [AlF3-OPO3] transition state analogue, Mg2+, and Na+
Method: X-RAY DIFFRACTION / Resolution: 1.698 Å

PDB-4dlc:
Crystal Structure of Trypanosoma brucei dUTPase with dUMP, MgF3- transition state analogue, and Mg2+
Method: X-RAY DIFFRACTION / Resolution: 1.759 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-DUN:
2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-DIPHOSPHATE

ChemComp-CA:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-UMP:
2'-DEOXYURIDINE 5'-MONOPHOSPHATE

ChemComp-AF3:
ALUMINUM FLUORIDE

ChemComp-PO4:
PHOSPHATE ION

ChemComp-MG:
Unknown entry

ChemComp-MGF:
TRIFLUOROMAGNESATE

Source
  • trypanosoma brucei (eukaryote)
KeywordsHYDROLASE / all alpha NTP pyrophosphohydrolase / all alpha NTP pyrophosphatase / dUTP and Mg2+ binding / HYDROLASE/HYDROLASE INHIBITOR / HYDROLASE-HYDROLASE INHIBITOR complex

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