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Structure paper

TitleCrystal Structures of Human Soluble Adenylyl Cyclase Reveal Mechanisms of Catalysis and of its Activation Through Bicarbonate.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 111, Page 3727-, Year 2014
Publish dateJan 14, 2014 (structure data deposition date)
AuthorsKleinboelting, S. / Diaz, A. / Moniot, S. / Van Den Heuvel, J. / Weyand, M. / Levin, L.R. / Buck, J. / Steegborn, C.
External linksProc. Natl. Acad. Sci. USA / PubMed:24567411
MethodsX-ray diffraction
Resolution1.7 - 3.2 Å
Structure data

PDB-4clf:
Crystal structure of human soluble Adenylyl Cyclase (Apo form)
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-4clk:
Crystal structure of human soluble Adenylyl Cyclase in complex with alpha,beta-methyleneadenosine-5'-triphosphate
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-4cll:
Crystal structure of human soluble Adenylyl Cyclase in complex with bicarbonate
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-4clp:
Crystal structure of human soluble Adenylyl Cyclase complex with adenosine-3',5'-cyclic-monophosphate
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4cls:
Crystal structure of human soluble Adenylyl Cyclase with Pyrophosphate
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-4clt:
Crystal structure of human soluble Adenylyl Cyclase with adenosine-3', 5'-cyclic-monophosphate and pyrophosphate
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-4clu:
Crystal structure of human soluble Adenylyl Cyclase with pyrophosphate
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4clw:
Crystal structure of human soluble Adenylyl Cyclase in complex with alpha,beta-methyleneadenosine-5'-triphosphate soaked with bisulfite
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-4cly:
Crystal structure of human soluble Adenylyl Cyclase soaked with biselenite
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-4clz:
Crystal structure of human soluble Adenylyl Cyclase with Inhibitor 4, 4'-Diisothiocyano-2,2'-stilbenedisulfonic acid
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4cm0:
Crystal structure of human soluble Adenylyl Cyclase with alpha,beta- methyleneadenosine-5'-triphosphate soaked with bicarbonate
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-4cm2:
Crystal structure of human soluble Adenylyl Cyclase soaked with bisulfite
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-ACT:
ACETATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-APC:
DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER / AMP-CPP, energy-carrying molecule analogue*YM

ChemComp-CL:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

ChemComp-CMP:
ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-MG:
Unknown entry

ChemComp-POP:
PYROPHOSPHATE 2-

ChemComp-SO3:
SULFITE ION

ChemComp-BSY:
BISELENITE ION

ChemComp-4DS:
4,4'-Diisothiocyano-2,2'-stilbenedisulfonic acid / inhibitor*YM

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

Source
  • homo sapiens (human)
KeywordsLYASE / ADENOSINE-3'\ / 5'-CYCLIC-MONOPHOSPHATE / ADENOSINE-5'-TRIPHOSPHATE / ACTIVATION / NUCLEOTIDE-BINDING / REACTION / BICARBONATE / ACTIVATOR

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