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TitleBiochemical Analysis and Structure Determination of Paucimonas Lemoignei Poly(3-Hydroxybutyrate) (Phb) Depolymerase Phaz7 Muteins Reveal the Phb Binding Site and Details of Substrate-Enzyme Interactions.
Journal, issue, pagesMol. Microbiol., Vol. 90, Page 649-, Year 2013
Publish dateJun 5, 2013 (structure data deposition date)
AuthorsJendrossek, D. / Hermawan, S. / Subedi, B. / Papageorgiou, A.C.
External linksMol. Microbiol. / PubMed:24007310
MethodsX-ray diffraction
Resolution1.594 - 2.002 Å
Structure data

PDB-4brs:
Structure of wild type PhaZ7 PHB depolymerase
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-4btv:
Structure of PhaZ7 PHB depolymerase in complex with 3HB trimer
Method: X-RAY DIFFRACTION / Resolution: 1.594 Å

PDB-4bvj:
Structure of Y105A mutant of PhaZ7 PHB depolymerase
Method: X-RAY DIFFRACTION / Resolution: 1.599 Å

PDB-4bvk:
Structure of Y190E mutant of PhaZ7 PHB depolymerase
Method: X-RAY DIFFRACTION / Resolution: 1.606 Å

PDB-4bvl:
Structure of 202-208 deletion mutant of PhaZ7 PHB depolymerase
Method: X-RAY DIFFRACTION / Resolution: 2.002 Å

PDB-4bym:
Structure of PhaZ7 PHB depolymerase Y105E mutant
Method: X-RAY DIFFRACTION / Resolution: 1.598 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-RB3:
(1R)-3-{[(1R)-3-METHOXY-1-METHYL-3-OXOPROPYL]OXY}-1-METHYL-3-OXOPROPYL (3R)-3-HYDROXYBUTANOATE

Source
  • paucimonas lemoignei (bacteria)
KeywordsHYDROLASE / CATALYTIC TRIAD / BIOPOLYMER BINDING / SUBSTRATE-BINDING CAVITY / BINDING CAVITY / 3HB TRIMER BINDING

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