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Structure paper

TitleCrystal Structure of Alpha-1,4-Glucan Lyase, a Unique Glycoside Hydrolase Family Member with a Novel Catalytic Mechanism.
Journal, issue, pagesJ. Biol. Chem., Vol. 288, Page 26764-, Year 2013
Publish dateJan 13, 2010 (structure data deposition date)
AuthorsRozeboom, H.J. / Yu, S. / Madrid, S. / Kalk, K.H. / Zhang, R. / Dijkstra, B.W.
External linksJ. Biol. Chem. / PubMed:23902768
MethodsX-ray diffraction
Resolution1.9 - 2.6 Å
Structure data

PDB-2x2h:
Crystal structure of the Gracilariopsis lemaneiformis alpha-1,4- glucan lyase
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

PDB-2x2i:
Crystal structure of the Gracilariopsis lemaneiformis alpha-1,4- glucan lyase with acarbose
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-2x2j:
Crystal structure of the Gracilariopsis lemaneiformis alpha- 1,4-glucan lyase with deoxynojirimycin
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-4amw:
CRYSTAL STRUCTURE OF THE GRACILARIOPSIS LEMANEIFORMIS ALPHA-1,4- GLUCAN LYASE Covalent Intermediate Complex with 5-fluoro-idosyl- fluoride
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4amx:
CRYSTAL STRUCTURE OF THE GRACILARIOPSIS LEMANEIFORMIS ALPHA-1,4- GLUCAN LYASE Covalent Intermediate Complex with 5-fluoro-glucosyl- fluoride
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-ACT:
ACETATE ION

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-NOJ:
1-DEOXYNOJIRIMYCIN

ChemComp-B9D:
5-fluoro-alpha-L-idopyranose

ChemComp-5DI:
alpha-D-threo-hexo-2,5-diulo-2,6-pyranosyl fluoride

ChemComp-5GF:
5-fluoro-beta-D-glucopyranose

ChemComp-AFR:
alpha-D-threo-hexo-2,5-diulo-5,1-pyranosyl fluoride

Source
  • gracilariopsis lemaneiformis (eukaryote)
KeywordsLYASE / ANHYDROFRUCTOSE PATHWAY / GLYCOSIDE HYDROLASE FAMILY 31 / STARCH BINDING DOMAIN / SECONDARY CARBOHYDRATE BINDING SITE

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