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TitleStructural insights into methyltransferase KsgA function in 30S ribosomal subunit biogenesis.
Journal, issue, pagesJ Biol Chem, Vol. 287, Issue 13, Page 10453-10459, Year 2012
Publish dateMar 23, 2012
AuthorsDaniel Boehringer / Heather C O'Farrell / Jason P Rife / Nenad Ban /
PubMed AbstractThe assembly of the ribosomal subunits is facilitated by ribosome biogenesis factors. The universally conserved methyltransferase KsgA modifies two adjacent adenosine residues in the 3'-terminal ...The assembly of the ribosomal subunits is facilitated by ribosome biogenesis factors. The universally conserved methyltransferase KsgA modifies two adjacent adenosine residues in the 3'-terminal helix 45 of the 16 S ribosomal RNA (rRNA). KsgA recognizes its substrate adenosine residues only in the context of a near mature 30S subunit and is required for the efficient processing of the rRNA termini during ribosome biogenesis. Here, we present the cryo-EM structure of KsgA bound to a nonmethylated 30S ribosomal subunit. The structure reveals that KsgA binds to the 30S platform with the catalytic N-terminal domain interacting with substrate adenosine residues in helix 45 and the C-terminal domain making extensive contacts to helix 27 and helix 24. KsgA excludes the penultimate rRNA helix 44 from adopting its position in the mature 30S subunit, blocking the formation of the decoding site and subunit joining. We suggest that the activation of methyltransferase activity and subsequent dissociation of KsgA control conformational changes in helix 44 required for final rRNA processing and translation initiation.
External linksJ Biol Chem / PubMed:22308031 / PubMed Central
MethodsEM (single particle)
Resolution13.5 - 17.7 Å
Structure data

2017

4adv

EMDB-2017, PDB-4adv:
Structure of the E. coli methyltransferase KsgA bound to the E. coli 30S ribosomal subunit
Method: EM (single particle) / Resolution: 13.5 Å

EMDB-2019:
Structure of the E. coli 30S ribosomal subunit from a ksgA deletion strain
Method: EM (single particle) / Resolution: 15.5 Å

EMDB-2020:
Structure of the E. coli 30S ribosomal subunit from a ksgA deletion strain in the conformation obtained at high magnesium concentrations
Method: EM (single particle) / Resolution: 17.7 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
KeywordsTRANSLATION / RIBOSOME BIOGENESIS / SMALL RIBOSOMAL SUBUNIT

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