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Structure paper

TitleA new structural form in the SAM/metal-dependent o‑methyltransferase family: MycE from the mycinamicin biosynthetic pathway.
Journal, issue, pagesJ. Mol. Biol., Vol. 413, Page 438-450, Year 2011
Publish dateJul 8, 2011 (structure data deposition date)
AuthorsAkey, D.L. / Li, S. / Konwerski, J.R. / Confer, L.A. / Bernard, S.M. / Anzai, Y. / Kato, F. / Sherman, D.H. / Smith, J.L.
External linksJ. Mol. Biol. / PubMed:21884704
MethodsX-ray diffraction
Resolution1.895 - 2.392 Å
Structure data

PDB-3ssm:
MycE Methyltransferase from the Mycinamycin Biosynthetic Pathway in Complex with Mg and SAH, Crystal form 1
Method: X-RAY DIFFRACTION / Resolution: 2.247 Å

PDB-3ssn:
MycE Methyltransferase from the Mycinamycin Biosynthetic Pathway in Complex with Mg, SAH, and Mycinamycin VI
Method: X-RAY DIFFRACTION / Resolution: 2.392 Å

PDB-3sso:
MycE Methyltransferase from the Mycinamycin Biosynthetic Pathway in Complex with Mg and SAH, Crystal form 2
Method: X-RAY DIFFRACTION / Resolution: 1.895 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-HOH:
WATER

ChemComp-MVI:
Mycinamicin VI

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

ChemComp-GOL:
GLYCEROL

Source
  • micromonospora griseorubida (bacteria)
KeywordsTRANSFERASE / methyltransferase / macrolide / natural product / Rossmann Fold / TRANSFERASE/ANTIBIOTIC / TRANSFERASE-ANTIBIOTIC complex

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