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Structure paper

TitleComputation-guided backbone grafting of a discontinuous motif onto a protein scaffold.
Journal, issue, pagesScience, Vol. 334, Page 373-376, Year 2011
Publish dateApr 27, 2011 (structure data deposition date)
AuthorsAzoitei, M.L. / Correia, B.E. / Ban, Y.E. / Carrico, C. / Kalyuzhniy, O. / Chen, L. / Schroeter, A. / Huang, P.S. / McLellan, J.S. / Kwong, P.D. ...Azoitei, M.L. / Correia, B.E. / Ban, Y.E. / Carrico, C. / Kalyuzhniy, O. / Chen, L. / Schroeter, A. / Huang, P.S. / McLellan, J.S. / Kwong, P.D. / Baker, D. / Strong, R.K. / Schief, W.R.
External linksScience / PubMed:22021856
MethodsX-ray diffraction
Resolution1.303 - 2.07 Å
Structure data

PDB-3rpt:
Crystal structure of the anti-HIV b12 scaffold protein
Method: X-RAY DIFFRACTION / Resolution: 1.303 Å

PDB-3ru8:
Structure of an HIV epitope scaffold in complex with neutralizing antibody b12 Fab
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

Source
  • thermobifida fusca (bacteria)
  • human immunodeficiency virus
  • synthetic construct (others)
  • thermomonospora fusca (bacteria)
  • homo sapiens (human)
KeywordsHYDROLASE / scaffold protein anti-HIV / DE NOVO PROTEIN / IMMUNE SYSTEM / binding epitope from HIV on a heterologous protein

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