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Structure paper

TitleCrystal Structure of Uronate Dehydrogenase from Agrobacterium tumefaciens.
Journal, issue, pagesJ. Biol. Chem., Vol. 286, Page 27294-27300, Year 2011
Publish dateApr 7, 2011 (structure data deposition date)
AuthorsParkkinen, T. / Boer, H. / Janis, J. / Andberg, M. / Penttila, M. / Koivula, A. / Rouvinen, J.
External linksJ. Biol. Chem. / PubMed:21676870
MethodsX-ray diffraction
Resolution1.9 - 2.1 Å
Structure data

PDB-3rft:
Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-3rfv:
Crystal structure of Uronate dehydrogenase from Agrobacterium tumefaciens complexed with NADH and product
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-3rfx:
Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens, Y136A mutant complexed with NAD
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-NAI:
1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE

ChemComp-15L:
D-galactaro-1,5-lactone

ChemComp-PO4:
PHOSPHATE ION

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

Source
  • agrobacterium tumefaciens (bacteria)
KeywordsOXIDOREDUCTASE / apoenzyme / Rossmann Fold / NAD binding / ternary complex / active site mutant / NAD

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