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Structure paper

TitleLigand Binding Induces an Ammonia Channel in 2-Amino-2-desoxyisochorismate (ADIC) Synthase PhzE.
Journal, issue, pagesJ. Biol. Chem., Vol. 286, Page 18213-18221, Year 2011
Publish dateMar 22, 2011 (structure data deposition date)
AuthorsLi, Q.A. / Mavrodi, D.V. / Thomashow, L.S. / Roessle, M. / Blankenfeldt, W.
External linksJ. Biol. Chem. / PubMed:21454481
MethodsX-ray diffraction
Resolution1.9 - 2.9 Å
Structure data

PDB-3r74:
Crystal structure of 2-amino-2-desoxyisochorismate synthase (ADIC) synthase PhzE from Burkholderia lata 383
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-3r75:
Crystal structure of 2-amino-2-desoxyisochorismate synthase (ADIC) synthase PhzE from Burkholderia lata 383 in complex with benzoate, pyruvate, glutamine and contaminating Zn2+
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-3r76:
Crystal structure of 2-amino-2-desoxyisochorismate synthase (ADIC) synthase PhzE from Burkholderia lata 383 in complex with benzoate, pyruvate and glutamine
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-3r77:
Crystal structure of the D38A mutant of isochorismatase PhzD from Pseudomonas fluorescens 2-79 in complex with 2-amino-2-desoxyisochorismate ADIC
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-BEZ:
BENZOIC ACID

ChemComp-PYR:
PYRUVIC ACID

ChemComp-PO4:
PHOSPHATE ION

ChemComp-ZN:
Unknown entry

ChemComp-QLI:
(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylic acid

ChemComp-CL:
Unknown entry

Source
  • burkholderia sp. (bacteria)
  • pseudomonas fluorescens (bacteria)
KeywordsLYASE / BIOSYNTHETIC PROTEIN / ammonia channel / chorismate / type 1 glutamine amidotransferase / phenazine biosynthesis / SYNTHASE / HYDROLASE / isochorismatase

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