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Structure paper

TitleCharacterization of member of DUF1888 protein family, self-cleaving and self-assembling endopeptidase.
Journal, issue, pagesJ. Biol. Chem., Vol. 287, Page 19452-19461, Year 2012
Publish dateMay 24, 2010 (structure data deposition date)
AuthorsOsipiuk, J. / Mulligan, R. / Bargassa, M. / Hamilton, J.E. / Cunningham, M.A. / Joachimiak, A.
External linksJ. Biol. Chem. / PubMed:22493430
MethodsX-ray diffraction
Resolution1.25 - 2.47 Å
Structure data

PDB-3n55:
SO1698 protein, an aspartic peptidase from Shewanella oneidensis.
Method: X-RAY DIFFRACTION / Resolution: 1.57 Å

PDB-3njf:
Y26F mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis
Method: X-RAY DIFFRACTION / Resolution: 2.47 Å

PDB-3njg:
K98A mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis.
Method: X-RAY DIFFRACTION / Resolution: 1.92 Å

PDB-3njh:
D37A mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis.
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

PDB-3nji:
S114A mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis.
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-3njj:
P115A mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis
Method: X-RAY DIFFRACTION / Resolution: 1.56 Å

PDB-3njk:
D116A mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis, at pH5.5
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-3njl:
D116A mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis, at pH7.5
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-3njm:
P117A mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis.
Method: X-RAY DIFFRACTION / Resolution: 1.64 Å

PDB-3njn:
Q118A mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis
Method: X-RAY DIFFRACTION / Resolution: 1.25 Å

Chemicals

ChemComp-BME:
BETA-MERCAPTOETHANOL

ChemComp-ACT:
ACETATE ION

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-CA:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-MG:
Unknown entry

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

Source
  • shewanella oneidensis (bacteria)
KeywordsHYDROLASE / structural genomics / aspartic peptidase / autocatalysis / PSI-2 / Protein Structure Initiative / Midwest Center for Structural Genomics / MCSG

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