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Structure paper

TitleConformational changes in orotidine 5'-monophosphate decarboxylase: "remote" residues that stabilize the active conformation.
Journal, issue, pagesBiochemistry, Vol. 49, Page 3514-3516, Year 2010
Publish dateJan 23, 2010 (structure data deposition date)
AuthorsWood, B.M. / Amyes, T.L. / Fedorov, A.A. / Fedorov, E.V. / Shabila, A. / Almo, S.C. / Richard, J.P. / Gerlt, J.A.
External linksBiochemistry / PubMed:20369850
MethodsX-ray diffraction
Resolution1.2 - 1.6 Å
Structure data

PDB-3lht:
Crystal structure of the mutant V201F of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-3lhu:
Crystal structure of the mutant I199F of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-3lhv:
Crystal structure of the mutant V182A.I199A.V201A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-3lhw:
Crystal structure of the mutant V182A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-3lhy:
Crystal structure of the mutant I199A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-3lhz:
Crystal structure of the mutant V201A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-3li1:
Crystal structure of the mutant I218A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-3lld:
Crystal structure of the mutant S127G of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with 6-azauridine 5'-monophosphate
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

PDB-3llf:
Crystal structure of the mutant S127P of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with 6-azauridine 5'-monophosphate
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-3ltp:
Crystal structure of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-3lts:
Crystal structure of the mutant V182A,I199A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.428 Å

PDB-3lty:
Crystal structure of the mutant V182A,I218A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-3lv5:
Crystal structure of the mutant I199E of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.444 Å

PDB-3lv6:
Crystal structure of the mutant I218F of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.451 Å

PDB-3m1z:
Crystal structure of the mutant V182A.V201A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP
Method: X-RAY DIFFRACTION / Resolution: 1.42 Å

PDB-3m41:
Crystal structure of the mutant V182A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-3m43:
Crystal structure of the mutant I199A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-3m44:
Crystal structure of the mutant V201A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-3m47:
Crystal structure of the mutant I218A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum
Method: X-RAY DIFFRACTION / Resolution: 1.2 Å

PDB-3m5x:
Crystal structure of the mutant V182A,I199A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-3m5y:
Crystal structure of the mutant V182A,V201A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum
Method: X-RAY DIFFRACTION / Resolution: 1.455 Å

PDB-3m5z:
Crystal structure of the mutant V182A,I218A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

Chemicals

ChemComp-BMQ:
1-(5'-PHOSPHO-BETA-D-RIBOFURANOSYL)BARBITURIC ACID

ChemComp-HOH:
WATER

ChemComp-UP6:
6-AZA URIDINE 5'-MONOPHOSPHATE

ChemComp-BMP:
6-HYDROXYURIDINE-5'-PHOSPHATE

ChemComp-GOL:
GLYCEROL

ChemComp-FMT:
FORMIC ACID

ChemComp-SO4:
SULFATE ION

Source
  • methanothermobacter thermautotrophicus (archaea)
  • methanothermobacter thermautotrophicus str. delta h (archaea)
KeywordsLYASE / mutant V201F / 6-hydroxyuridine-5'-phosphate / Decarboxylase / Pyrimidine biosynthesis / mutant I199F / mutant V182A.I199A.V201A / mutant V182A / mutant I199A / mutant I218A / orotidine 5'-monophosphate decarboxylase / S127G / 6-azauridine 5'-monophosphate / S127P / inhibitor BMP / I199A / mutant V182A.I218A / mutant I199E / mutant I218F / BMP / mutant V201A / V201A / I218A

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