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TitleHigh-resolution microtubule structures reveal the structural transitions in αβ-tubulin upon GTP hydrolysis.
Journal, issue, pagesCell, Vol. 157, Issue 5, Page 1117-1129, Year 2014
Publish dateMay 22, 2014
AuthorsGregory M Alushin / Gabriel C Lander / Elizabeth H Kellogg / Rui Zhang / David Baker / Eva Nogales /
PubMed AbstractDynamic instability, the stochastic switching between growth and shrinkage, is essential for microtubule function. This behavior is driven by GTP hydrolysis in the microtubule lattice and is ...Dynamic instability, the stochastic switching between growth and shrinkage, is essential for microtubule function. This behavior is driven by GTP hydrolysis in the microtubule lattice and is inhibited by anticancer agents like Taxol. We provide insight into the mechanism of dynamic instability, based on high-resolution cryo-EM structures (4.7-5.6 Å) of dynamic microtubules and microtubules stabilized by GMPCPP or Taxol. We infer that hydrolysis leads to a compaction around the E-site nucleotide at longitudinal interfaces, as well as movement of the α-tubulin intermediate domain and H7 helix. Displacement of the C-terminal helices in both α- and β-tubulin subunits suggests an effect on interactions with binding partners that contact this region. Taxol inhibits most of these conformational changes, allosterically inducing a GMPCPP-like state. Lateral interactions are similar in all conditions we examined, suggesting that microtubule lattice stability is primarily modulated at longitudinal interfaces.
External linksCell / PubMed:24855948 / PubMed Central
MethodsEM (single particle)
Resolution4.7 - 6.6 Å
Structure data

5895

3j6e

EMDB-5895: Cryo EM density of microtubules stabilized by GMPCPP
PDB-3j6e: Energy minimized average structure of Microtubules stabilized by GmpCpp
Method: EM (single particle) / Resolution: 4.7 Å

5896

3j6f

EMDB-5896: Cryo EM density of GDP-bound dynamic microtubules
PDB-3j6f: Minimized average structure of GDP-bound dynamic microtubules
Method: EM (single particle) / Resolution: 4.9 Å

5897

3j6g

EMDB-5897: Cryo EM density of microtubules stabilized by taxol
PDB-3j6g: Minimized average structure of microtubules stabilized by taxol
Method: EM (single particle) / Resolution: 5.5 Å

EMDB-5898:
Cryo EM density of microtubules stabilized by GMPCPP, without kinesin
Method: EM (single particle) / Resolution: 6.6 Å

EMDB-5899:
Cryo EM density of microtubules bound to GDP, no kinesin
Method: EM (single particle) / Resolution: 6.3 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

ChemComp-HOH:
WATER / Water

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM / Paclitaxel

Source
  • sus scrofa (pig)
  • Homo sapiens (human)
KeywordsSTRUCTURAL PROTEIN / microtubule / GmpCpp / GDP / dynamic / taxol

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