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TitleStructure of Sputnik, a virophage, at 3.5-Å resolution.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 109, Issue 45, Page 18431-18436, Year 2012
Publish dateNov 6, 2012
AuthorsXinzheng Zhang / Siyang Sun / Ye Xiang / Jimson Wong / Thomas Klose / Didier Raoult / Michael G Rossmann /
PubMed Abstract"Sputnik" is a dsDNA virus, referred to as a virophage, that is coassembled with Mimivirus in the host amoeba. We have used cryo-EM to produce an electron density map of the icosahedral Sputnik virus ..."Sputnik" is a dsDNA virus, referred to as a virophage, that is coassembled with Mimivirus in the host amoeba. We have used cryo-EM to produce an electron density map of the icosahedral Sputnik virus at 3.5-Å resolution, sufficient to verify the identity of most amino acids in the capsid proteins and to establish the identity of the pentameric protein forming the fivefold vertices. It was also shown that the virus lacks an internal membrane. The capsid is organized into a T = 27 lattice in which there are 260 trimeric capsomers and 12 pentameric capsomers. The trimeric capsomers consist of three double "jelly-roll" major capsid proteins creating pseudohexameric capsomer symmetry. The pentameric capsomers consist of five single jelly-roll proteins. The release of the genome by displacing one or more of the pentameric capsomers may be the result of a low-pH environment. These results suggest a mechanism of Sputnik DNA ejection that probably also occurs in other big icosahedral double jelly-roll viruses such as Adenovirus. In this study, the near-atomic resolution structure of a virus has been established where crystallization for X-ray crystallography was not feasible.
External linksProc Natl Acad Sci U S A / PubMed:23091035 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 3.8 Å
Structure data

5495

3j26

EMDB-5495: Cryo-EM reconstruction of Sputnik virus
PDB-3j26: The 3.5 A resolution structure of the Sputnik virophage by cryo-EM
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-5496:
Cryo-EM reconstruction of Sputnik empty virus
Method: EM (single particle) / Resolution: 3.8 Å

Source
  • sputnik virophage (virus)
KeywordsVIRUS / double jelly-roll / single jelly-roll

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