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TitleStructure of the no-go mRNA decay complex Dom34-Hbs1 bound to a stalled 80S ribosome.
Journal, issue, pagesNat Struct Mol Biol, Vol. 18, Issue 6, Page 715-720, Year 2011
Publish dateMay 29, 2011
AuthorsThomas Becker / Jean-Paul Armache / Alexander Jarasch / Andreas M Anger / Elizabeth Villa / Heidemarie Sieber / Basma Abdel Motaal / Thorsten Mielke / Otto Berninghausen / Roland Beckmann /
PubMed AbstractNo-go decay (NGD) is a mRNA quality-control mechanism in eukaryotic cells that leads to degradation of mRNAs stalled during translational elongation. The key factors triggering NGD are Dom34 and Hbs1. ...No-go decay (NGD) is a mRNA quality-control mechanism in eukaryotic cells that leads to degradation of mRNAs stalled during translational elongation. The key factors triggering NGD are Dom34 and Hbs1. We used cryo-EM to visualize NGD intermediates resulting from binding of the Dom34-Hbs1 complex to stalled ribosomes. At subnanometer resolution, all domains of Dom34 and Hbs1 were identified, allowing the docking of crystal structures and homology models. Moreover, the close structural similarity of Dom34 and Hbs1 to eukaryotic release factors (eRFs) enabled us to propose a model for the ribosome-bound eRF1-eRF3 complex. Collectively, our data provide structural insights into how stalled mRNA is recognized on the ribosome and how the eRF complex can simultaneously recognize stop codons and catalyze peptide release.
External linksNat Struct Mol Biol / PubMed:21623367
MethodsEM (single particle)
Resolution9.4 - 27.0 Å
Structure data

EMDB-1808:
Yeast 80S ribosome stalled by a stem-loop containing mRNA.
Method: EM (single particle) / Resolution: 12.1 Å

EMDB-1809:
Yeast 80S ribosome stalled by a stem-loop containing mRNA in complex with Dom34p and N-terminally truncated Hbs1p.
Method: EM (single particle) / Resolution: 27.0 Å

1811

3izq

EMDB-1811: Yeast 80S ribosome stalled by a stem-loop containing mRNA in complex with Dom34-Hbs1. The dataset is computationally sorted for presence of P-site tRNA and Dom34-Hbs1.
PDB-3izq: Structure of the Dom34-Hbs1-GDPNP complex bound to a translating ribosome
Method: EM (single particle) / Resolution: 9.5 Å

EMDB-1812:
Yeast 80S ribosome stalled by a stem-loop containing mRNA in complex with Dom34-Hbs1. The dataset is computationally sorted for absence of P-site tRNA and the presence of Dom34-Hbs1 complex.
Method: EM (single particle) / Resolution: 9.4 Å

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsRIBOSOMAL PROTEIN / HYDROLASE / No-Go mRNA decay

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