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Structure paper

TitleDirected evolution of an enantioselective epoxide hydrolase: uncovering the source of enantioselectivity at each evolutionary stage
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 131, Page 7334-7343, Year 2009
Publish dateJan 27, 2009 (structure data deposition date)
AuthorsReetz, M.T. / Bocola, M. / Wang, L.-W. / Sanchis, J. / Cronin, A. / Arand, M. / Zou, J. / Archelas, A. / Bottalla, A.-L. / Naworyta, A. / Mowbray, S.L.
External linksJ. Am. Chem. Soc. / PubMed:19469578
MethodsX-ray diffraction
Resolution1.5 - 2.1 Å
Structure data

PDB-3g02:
Structure of enantioselective mutant of epoxide hydrolase from Aspergillus niger generated by directed evolution
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-3g0i:
Complex of Aspergillus niger epoxide hydrolase with valpromide (2-propylpentanamide)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-FMT:
FORMIC ACID

ChemComp-HOH:
WATER

ChemComp-VPR:
2-PROPYLPENTANAMIDE

Source
  • aspergillus niger (mold)
KeywordsHYDROLASE / EPOXIDE HYDROLASE / ALPHA/BETA HYDROLASE FOLD / enantioselective / mutant / directed evolution / valpromide / 2-propylpentanamide

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