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Structure paper

TitleToward an antitumor form of bovine pancreatic ribonuclease: The crystal structure of three noncovalent dimeric mutants
Journal, issue, pagesBiopolymers, Vol. 91, Page 1029-1037, Year 2009
Publish dateDec 18, 2008 (structure data deposition date)
AuthorsMerlino, A. / Russo Krauss, I. / Perillo, M. / Mattia, C.A. / Ercole, C. / Picone, D. / Vergara, A. / Sica, F.
External linksBiopolymers / PubMed:19280639
MethodsX-ray diffraction
Resolution1.6 - 1.99 Å
Structure data

PDB-3fkz:
X-ray structure of the non covalent swapped form of the S16G/T17N/A19P/A20S/K31C/S32C mutant of bovine pancreatic ribonuclease
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

PDB-3fl0:
X-ray structure of the non covalent swapped form of the Q28L/K31C/S32C mutant of bovine pancreatic ribonuclease in complex with 2'-DEOXYCYTIDINE-2'-DEOXYGUANOSINE-3',5'-MONOPHOSPHATE
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

PDB-3fl1:
X-ray structure of the non covalent swapped form of the A19P/Q28L/K31C/S32C mutant of bovine pancreatic ribonuclease in complex with 2'-DEOXYCYTIDINE-2'-DEOXYGUANOSINE-3',5'-MONOPHOSPHATE
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-3fl3:
X-ray structure of the ligand free non covalent swapped form of the A19P/Q28L/K31C/S32C mutant of bovine pancreatic ribonuclease
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-CGP:
2'-DEOXYCYTIDINE-2'-DEOXYGUANOSINE-3',5'-MONOPHOSPHATE

ChemComp-SO4:
SULFATE ION

Source
  • bos taurus (domestic cattle)
KeywordsHYDROLASE / 3D-domain swapping / bovine seminal ribonuclease / non-covalent dimer / antitumor activity / quaternary structure flexibility / protein mutations and evolution / Endonuclease / Glycation / Glycoprotein / Nuclease / Secreted

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