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Structure paper

TitleStructural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair.
Journal, issue, pagesFebs J., Vol. 276, Page 5610-5621, Year 2009
Publish dateDec 16, 2008 (structure data deposition date)
AuthorsKlieber, M.A. / Scholz, O. / Lochner, S. / Gmeiner, P. / Hillen, W. / Muller, Y.A.
External linksFebs J. / PubMed:19712110
MethodsX-ray diffraction
Resolution2.06 - 2.1 Å
Structure data

PDB-3fk6:
Crystal structure of TetR triple mutant (H64K, S135L, S138I)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-3fk7:
Crystal structure of TetR triple mutant (H64K, S135L, S138I) in complex with 4-ddma-atc
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-4DM:
(4aS,12aS)-3,10,11,12a-tetrahydroxy-6-methyl-1,12-dioxo-1,4,4a,5,12,12a-hexahydrotetracene-2-carboxamide

Source
  • escherichia coli (E. coli)
KeywordsTRANSCRIPTION / Tetracycline repressor / bacterial transcription regulation / altered inducer specificity / 4-de-dimethylamino-anhydrotetracycline / Antibiotic resistance / DNA-binding / Magnesium / Metal-binding / Repressor / Transcription regulation / Transposable element

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