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Structure paper

TitleATPase cycle of the nonmotile kinesin NOD allows microtubule end tracking and drives chromosome movement.
Journal, issue, pagesCell, Vol. 136, Issue 1, Page 110-122, Year 2009
Publish dateJan 9, 2009
AuthorsJared C Cochran / Charles V Sindelar / Natasha K Mulko / Kimberly A Collins / Stephanie E Kong / R Scott Hawley / F Jon Kull /
PubMed AbstractSegregation of nonexchange chromosomes during Drosophila melanogaster meiosis requires the proper function of NOD, a nonmotile kinesin-10. We have determined the X-ray crystal structure of the NOD ...Segregation of nonexchange chromosomes during Drosophila melanogaster meiosis requires the proper function of NOD, a nonmotile kinesin-10. We have determined the X-ray crystal structure of the NOD catalytic domain in the ADP- and AMPPNP-bound states. These structures reveal an alternate conformation of the microtubule binding region as well as a nucleotide-sensitive relay of hydrogen bonds at the active site. Additionally, a cryo-electron microscopy reconstruction of the nucleotide-free microtubule-NOD complex shows an atypical binding orientation. Thermodynamic studies show that NOD binds tightly to microtubules in the nucleotide-free state, yet other nucleotide states, including AMPPNP, are weakened. Our pre-steady-state kinetic analysis demonstrates that NOD interaction with microtubules occurs slowly with weak activation of ADP product release. Upon rapid substrate binding, NOD detaches from the microtubule prior to the rate-limiting step of ATP hydrolysis, which is also atypical for a kinesin. We propose a model for NOD's microtubule plus-end tracking that drives chromosome movement.
External linksCell / PubMed:19135893 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction / EM (single particle)
Resolution1.9 - 11.0 Å
Structure data

5038

3dco

EMDB-5038: 11-Angstrom cryo-EM reconstruction of nucleotide-free Nod complexed to the 13-protofilament microtubule.
PDB-3dco: Drosophila NOD (3DC4) and Bovine Tubulin (1JFF) Docked into the 11-Angstrom Cryo-EM Map of Nucleotide-Free NOD Complexed to the Microtubule
Method: EM (helical sym.) / Resolution: 11.0 Å

PDB-3dc4:
Crystal structure of the Drosophila kinesin family member NOD in complex with ADP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-3dcb:
Crystal structure of the Drosophila kinesin family member NOD in complex with AMPPNP
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-HOH:
WATER / Water

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-ZN:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM / Paclitaxel

Source
  • unidentified (others)
  • drosophila melanogaster (fruit fly)
  • bos taurus (cattle)
KeywordsMOTOR PROTEIN / kinesin / catalytic domain / ATPase / microtubule / ADP / nucleotide-binding protein / ATP-binding / Coiled coil / Nucleotide-binding / AMPPNP / cryo-EM / 3D reconstruction / tubulin / nucleotide-free

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