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Structure paper

TitleStructural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release.
Journal, issue, pagesCell, Vol. 143, Issue 2, Page 275-287, Year 2010
Publish dateOct 15, 2010
AuthorsKenji Murakami / Takuo Yasunaga / Taro Q P Noguchi / Yuki Gomibuchi / Kien X Ngo / Taro Q P Uyeda / Takeyuki Wakabayashi /
PubMed AbstractAssembled actin filaments support cellular signaling, intracellular trafficking, and cytokinesis. ATP hydrolysis triggered by actin assembly provides the structural cues for filament turnover in vivo. ...Assembled actin filaments support cellular signaling, intracellular trafficking, and cytokinesis. ATP hydrolysis triggered by actin assembly provides the structural cues for filament turnover in vivo. Here, we present the cryo-electron microscopic (cryo-EM) structure of filamentous actin (F-actin) in the presence of phosphate, with the visualization of some α-helical backbones and large side chains. A complete atomic model based on the EM map identified intermolecular interactions mediated by bound magnesium and phosphate ions. Comparison of the F-actin model with G-actin monomer crystal structures reveals a critical role for bending of the conserved proline-rich loop in triggering phosphate release following ATP hydrolysis. Crystal structures of G-actin show that mutations in this loop trap the catalytic site in two intermediate states of the ATPase cycle. The combined structural information allows us to propose a detailed molecular mechanism for the biochemical events, including actin polymerization and ATPase activation, critical for actin filament dynamics.
External linksCell / PubMed:20946985
MethodsEM (single particle) / X-ray diffraction
Resolution2.36 - 6.0 Å
Structure data

1674

3g37

EMDB-1674: The cryo-EM structure of actin filament in the presence of phosphate
PDB-3g37: Cryo-EM structure of actin filament in the presence of phosphate
Method: EM (single particle) / Resolution: 6.0 Å

PDB-3a5l:
Crystal Structure of a Dictyostelium P109A Mg2+-Actin in Complex with Human Gelsolin Segment 1
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-3a5m:
Crystal Structure of a Dictyostelium P109I Mg2+-Actin in Complex with Human Gelsolin Segment 1
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-3a5n:
Crystal Structure of a Dictyostelium P109A Ca2+-Actin in Complex with Human Gelsolin Segment 1
Method: X-RAY DIFFRACTION / Resolution: 2.36 Å

PDB-3a5o:
Crystal Structure of a Dictyostelium P109I Ca2+-Actin in Complex with Human Gelsolin Segment 1
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-PO4:
PHOSPHATE ION

Source
  • oryctolagus cuniculus (rabbit)
  • homo sapiens (human)
  • dictyostelium discoideum (eukaryote)
KeywordsCONTRACTILE PROTEIN / ACTIN / ADP / HYDROLYSIS / ACTIN CAPPING / ACTIN-BINDING / CYTOSKELETON / ATP-BINDING / NUCLEOTIDE-BINDING / STRUCTURAL PROTEIN / cell adhesion / cellular signaling / cytokinesis / muscle / cryo-EM / Methylation / Muscle protein / Phosphoprotein

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