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Structure paper

TitleRNA polymerase II elongation complex with the +1 nucleosome
Journal, issue, pagesTo Be Published, Year 2026
Publish dateApr 7, 2026 (structure data deposition date)
AuthorsZhan Y / Abril-Garrido J
External linksTo Be Published / Search PubMed
MethodsEM (single particle)
Resolution3.1 - 12.4 Å
Structure data

57364
EMDB Unreleased entry

EMDB-57364, PDB-29tk:
RNA polymerase II elongation complex with the +1 nucleosome
Method: EM (single particle) / Resolution: 3.9 Å


EMDB Unreleased entry

EMDB-57365: Consensus map for RNA Polymerase II elongation complex with the +1 nucleosome
Method: EM (single particle) / Resolution: 3.9 Å

57390
EMDB Unreleased entry

EMDB-57390, PDB-29vf:
RNA polymerase II initially transcribing complex with a 2-nt RNA and the +1 nucleosome
Method: EM (single particle) / Resolution: 7.5 Å


EMDB Unreleased entry

EMDB-57391: Focused refinement map of core Pol II
Method: EM (single particle) / Resolution: 3.27 Å


EMDB Unreleased entry

EMDB-57392: Focused refinement map of nucleosome in RNA polymerase II elongation complex with the +1 nucleosome
Method: EM (single particle) / Resolution: 3.71 Å


EMDB Unreleased entry

EMDB-57394: Focused refinement map of nucleosome of RNA polymerase II initially transcribing complex with a 2-nt RNA and the +1 nucleosome
Method: EM (single particle) / Resolution: 7.2 Å


EMDB Unreleased entry

EMDB-57395: Focused refinement map of cPIC of RNA polymerase II initially transcribing complex with a 2-nt RNA and the +1 nucleosome
Method: EM (single particle) / Resolution: 4.26 Å

57401
EMDB Unreleased entry

EMDB-57401: RNA polymerase II pre-initiation complex bound to ADP-BeF3 in the presence of the +1 nucleosome
PDB-29wd: RNA polymerase II initially transcribing complex with a 2-nt RNA and the +1 nucleosome
Method: EM (single particle) / Resolution: 4.2 Å


EMDB Unreleased entry

EMDB-57402: Focused refinement map of the nucleosome in RNA polymerase II pre-initiation complex bound to ADP-BeF3 in the presence of the +1 nucleosome
Method: EM (single particle) / Resolution: 4.1 Å


EMDB Unreleased entry

EMDB-57403: focused refinement map of core PIC in RNA polymerase II pre-initiation complex bound to ADP-BeF3 in the presence of the +1 nucleosome
Method: EM (single particle) / Resolution: 3.6 Å


EMDB Unreleased entry

EMDB-57406: Focused refinement map of TFIIH in RNA polymerase II pre-initiation complex bound to ADP-BeF3 in the presence of the +1 nucleosome
Method: EM (single particle) / Resolution: 4.2 Å


EMDB Unreleased entry

EMDB-57407: Focused refinement map of XPB in RNA polymerase II pre-initiation complex bound to ADP-BeF3 in the presence of the +1 nucleosome
Method: EM (single particle) / Resolution: 3.1 Å


EMDB Unreleased entry

EMDB-57541: Focused map of the +1 nucleosome (Closed complex)
Method: EM (single particle) / Resolution: 7.5 Å


EMDB Unreleased entry

EMDB-57544: Focused map of core RNA polymerase II pre-initiation complex (PIC) (Closed complex)
Method: EM (single particle) / Resolution: 4.4 Å


EMDB Unreleased entry

EMDB-57545: Focused map of general transcription factor IIH (Closed complex)
Method: EM (single particle) / Resolution: 11.1 Å


EMDB Unreleased entry

EMDB-57628: Focused map of transcription initiation factor IID (TFIID) (Closed complex)
Method: EM (single particle) / Resolution: 12.4 Å

57691
EMDB Unreleased entry

EMDB-57691, PDB-30ff:
TFIID-containing RNA polymerase II pre-initiation complex in the presence of the +1 nucleosome
Method: EM (single particle) / Resolution: 8.5 Å

57693
EMDB Unreleased entry

EMDB-57693, PDB-30fh:
RNA polymerase II initially transcribing complex with a 6-nt RNA and the +1 nucleosome
Method: EM (single particle) / Resolution: 7.6 Å


EMDB Unreleased entry

EMDB-57789: Focused map of the +1 nucleosome (ITC6)
Method: EM (single particle) / Resolution: 4.0 Å


EMDB Unreleased entry

EMDB-57793: Focused map of general transcription factor IIH (ITC6)
Method: EM (single particle) / Resolution: 9.0 Å


EMDB Unreleased entry

EMDB-57807: Focused map of core RNA polymerase II pre-initiation complex (PIC) (ITC6)
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

ChemComp-UNK:
UNKNOWN

ChemComp-2A1:
(2S)-2-aminopropan-1-ol

ChemComp-ALA:
ALANINE

Source
  • homo sapiens (human)
  • sus scrofa (pig)
  • xenopus laevis (African clawed frog)
  • unidentified adenovirus
  • synthetic construct (others)
KeywordsTRANSCRIPTION / RNA polymerase II / Mammalian PIC / +1 nucleosome / transcription initiation

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