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TitleStructure and biochemistry reveal substrate-modulated ComEC nuclease activity during DNA processing.
Journal, issue, pagesNucleic Acids Res, Vol. 54, Issue 16, Year 2026
Publish dateAug 24, 2026
AuthorsSophie Deselaers / Dianhong Wang / Tamino Cairoli / Pavel Afanasyev / Manuela K Hospenthal /
PubMed AbstractNatural transformation enables bacteria to internalize extracellular DNA, driving adaptation and the spread of antibiotic resistance. The membrane protein ComEC mediates translocation of single- ...Natural transformation enables bacteria to internalize extracellular DNA, driving adaptation and the spread of antibiotic resistance. The membrane protein ComEC mediates translocation of single-stranded DNA (ssDNA) across the cytoplasmic membrane while degrading the complementary strand, yet the structural basis of its activity remains incompletely defined. Here, we report a cryo-electron microscopy structure of full-length ComEC from Neomoorella carbonis in a pre-translocation state, revealing a three-domain architecture and a conserved transmembrane channel captured in a closed conformation. Structural analysis indicates that conformational rearrangements of channel-lining helices would be required to accommodate ssDNA. Biochemical assays show that, relative to the isolated β-lactamase-like domain, full-length ComEC degrades DNA more efficiently and exhibits position-dependent cleavage of phosphodiester bonds within the DNA substrate. Importantly, coating of the DNA by the periplasmic DNA receptor ComEA suppresses endonucleolytic cleavage and enhances 5'' terminal cleavage, thereby directing ComEC towards productive processing of transforming DNA during natural transformation.
External linksNucleic Acids Res / PubMed:42689412 / PubMed Central
MethodsEM (single particle)
Resolution4.1 - 4.2 Å
Structure data

57548

30bt

EMDB-57548, PDB-30bt:
Cryo-EM structure of Beta-lactamase-like domain from Neomoorella carbonis
Method: EM (single particle) / Resolution: 4.2 Å

57549

30bu

32ks

EMDB-57549, PDB-30bu:
Cryo-EM structure of ComEC from Neomoorella carbonis
PDB-32ks: Cryo-EM structure of full-length ComEC from Neomoorella carbonis
Method: EM (single particle) / Resolution: 4.1 Å

Source
  • neomoorella carbonis (bacteria)
KeywordsMEMBRANE PROTEIN / DNA translocation / Nuclease / DNA binding

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