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TitleThe Tautomeric Half-reaction of BphD, a C-C Bond Hydrolase: KINETIC AND STRUCTURAL EVIDENCE SUPPORTING A KEY ROLE FOR HISTIDINE 265 OF THE CATALYTIC TRIAD.
Journal, issue, pagesJ. Biol. Chem., Vol. 282, Page 19894-19904, Year 2007
Publish dateMay 8, 2007 (structure data deposition date)
AuthorsHorsman, G.P. / Bhowmik, S. / Seah, S.Y. / Kumar, P. / Bolin, J.T. / Eltis, L.D.
External linksJ. Biol. Chem. / PubMed:17442675
MethodsX-ray diffraction
Resolution1.57 - 2.3 Å
Structure data

PDB-2pu5:
Crystal Structure of a C-C bond hydrolase, BphD, from Burkholderia xenovorans LB400
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-2pu7:
Crystal Structure of S112A/H265A double mutant of a C-C hydrolase, BphD, from Burkholderia xenovorans LB400
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

PDB-2puh:
Crystal Structure of the S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

PDB-2puj:
Crystal Structure of the S112A/H265A double mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, in complex with its substrate HOPDA
Method: X-RAY DIFFRACTION / Resolution: 1.57 Å

PDB-2ri6:
Crystal Structure of S112A mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400
Method: X-RAY DIFFRACTION / Resolution: 1.68 Å

Chemicals

ChemComp-MLI:
MALONATE ION

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

ChemComp-HPK:
(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE

ChemComp-HPZ:
(2E,4E)-2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOIC ACID

Source
  • burkholderia xenovorans (bacteria)
KeywordsHYDROLASE / C-C Bond hydrolase / Structural Genomics / Aromatic hydrocarbons catabolism

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